紫花苜蓿bZIP基因家族的鉴定、 进化及表达分析
发布时间：2018-08-05 14:36
【摘要】：碱性亮氨酸拉链(bZIP)转录因子是真核生物转录因子中分布最广泛、最保守的一类蛋白。目前在许多植物中已发现大量的bZIP转录因子,这些bZIP转录因子成员广泛参与种子贮藏基因的表达、植物的生长发育、光信号传导、病害防御、生物和非生物胁迫应答以及ABA的敏感性等各种信号的反应。本研究首次从紫花苜蓿(Medicago sativa)全转录组水平鉴定出bZIP转录因子家族共包含138个基因,根据bZIP蛋白序列进行系统进化分析可以将其分为10类;对MsbZIP基因的系统进化分析表明该基因家族在分类上有很高的保守性。该转录因子家族的基因密码子偏好性分析表明,MsbZIP基因密码子偏好使用A/T碱基。此外,MsbZIP基因GO功能注释分析结果显示,138个MsbZIP基因最终分为23个GO分类,总体包括分子功能和生物学过程两类。相关性分析结果表明,共有372对基因表达相关性极显著(P0.01)。本研究可为紫花苜蓿bZIP转录因子功能特性、进化历程和生物功能的深入研究奠定基础。
[Abstract]:Basic leucine zipper (bZIP) transcription factor is the most widely distributed and conserved class of eukaryotic transcription factors. At present, a large number of bZIP transcription factors have been found in many plants. These bZIP transcription factors are widely involved in the expression of seed storage genes, plant growth and development, light signal transduction, disease defense. Biological and abiotic stress response and ABA sensitivity and other signal responses. In this study, the bZIP transcription factor family was identified for the first time from the (Medicago sativa) full transcriptome level of alfalfa. The bZIP transcription factor family contains 138 genes, which can be classified into 10 categories by phylogenetic analysis of bZIP protein sequences. Phylogenetic analysis of MsbZIP gene showed that the gene family was highly conserved in classification. The codon preference analysis of the transcription factor family showed that the codon of MsbZIP gene preferred to use the A / T base. In addition, the results of go functional annotation analysis of MsbZIP gene showed that 138 MsbZIP genes were classified into 23 go categories, including molecular function and biological process. The results of correlation analysis showed that 372 pairs of genes were significantly correlated (P0.01). This study can lay a foundation for the further study of the functional characteristics, evolutionary process and biological functions of bZIP transcription factors in alfalfa.
【作者单位】： 中国农业科学院草原研究所;全国畜牧总站全国草品种审定委员会办公室;草地农业生态系统国家重点实验室兰州大学草地农业科技学院;
【基金】：现代农业产业技术体系建设专项资金国家牧草产业技术体系项目(CARS-35) 中国农业科学院科技创新工程牧草栽培与加工利用团队项目(CAAS-ASTIP-IGR 2015-02) 国家自然科学基金青年科学基金(31502000) 兰州大学中央高校基本科研业务费专项资金(lzujbky-2016-8) 国家草品种区域试验项目(21301060001) 巴彦淖尔肉羊优质饲草高效生产关键技术集成与研究应用
【分类号】：S541.9
本文编号：2166087
[Abstract]:Basic leucine zipper (bZIP) transcription factor is the most widely distributed and conserved class of eukaryotic transcription factors. At present, a large number of bZIP transcription factors have been found in many plants. These bZIP transcription factors are widely involved in the expression of seed storage genes, plant growth and development, light signal transduction, disease defense. Biological and abiotic stress response and ABA sensitivity and other signal responses. In this study, the bZIP transcription factor family was identified for the first time from the (Medicago sativa) full transcriptome level of alfalfa. The bZIP transcription factor family contains 138 genes, which can be classified into 10 categories by phylogenetic analysis of bZIP protein sequences. Phylogenetic analysis of MsbZIP gene showed that the gene family was highly conserved in classification. The codon preference analysis of the transcription factor family showed that the codon of MsbZIP gene preferred to use the A / T base. In addition, the results of go functional annotation analysis of MsbZIP gene showed that 138 MsbZIP genes were classified into 23 go categories, including molecular function and biological process. The results of correlation analysis showed that 372 pairs of genes were significantly correlated (P0.01). This study can lay a foundation for the further study of the functional characteristics, evolutionary process and biological functions of bZIP transcription factors in alfalfa.
【作者单位】： 中国农业科学院草原研究所;全国畜牧总站全国草品种审定委员会办公室;草地农业生态系统国家重点实验室兰州大学草地农业科技学院;
【基金】：现代农业产业技术体系建设专项资金国家牧草产业技术体系项目(CARS-35) 中国农业科学院科技创新工程牧草栽培与加工利用团队项目(CAAS-ASTIP-IGR 2015-02) 国家自然科学基金青年科学基金(31502000) 兰州大学中央高校基本科研业务费专项资金(lzujbky-2016-8) 国家草品种区域试验项目(21301060001) 巴彦淖尔肉羊优质饲草高效生产关键技术集成与研究应用
【分类号】：S541.9
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1 沈川;小麦内质网应激相关bZIP基因的鉴定与功能分析[D];西北农林科技大学;2016年
2 刘依;小麦热胁迫响应bZIP基因的克隆及功能鉴定[D];武汉工业学院;2012年
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