草莓钼转运蛋白基因MOT1的克隆与表达以及对氮代谢的影响
发布时间:2018-08-05 20:28
【摘要】:从‘章姬’草莓中克隆了一个MOT1家族成员的全长,测序发现其CDS序列与公布的森林草莓基因组序列一致,将其命名为FaMOT1。进化树分析表明Fa MOT1所编码的氨基酸与苹果和桃亲缘关系最近。荧光定量PCR结果表明,FaMOT1基因在草莓的根、茎、叶、花和10 d幼果中有不同程度的表达。对草莓幼苗喷施不同浓度的钼酸钠,结果发现,各处理叶片的钼浓度随着施钼量的增加而显著提高。2.4 mmol·L~(-1) Na2Mo O4处理显著诱导根部和叶片的FaMOT1的表达,处理2和4 h时4.8 mmol·L~(-1) Na_2MoO_4处理的茎部FaMOT1表达水平最高,处理6和12 h时2.4 mmol·L~(-1) Na_2MoO_4处理的茎部FaMOT1表达水平最高。2.4 mmol·L~(-1) Na_2MoO_4处理叶片的硝态氮浓度显著低于其它处理,氨态氮浓度显著高于其它处理;4.8 mmol·L~(-1)Na_2MoO_4处理根部和叶片的氨态氮浓度最低。以上结果表明FaMOT1基因的表达受到钼的诱导和调节,合适浓度的钼处理有利于硝态氮的吸收和向氨态氮的转化以及氨态氮向有机氮的转化。
[Abstract]:A member of MOT1 family was cloned from 'Zhang Ji' strawberry, and its CDS sequence was found to be consistent with the published genomic sequence of forest strawberry, which was named FaMOT 1. Phylogenetic tree analysis showed that the amino acids encoded by Fa MOT1 were most closely related to apple and peach. The results of fluorescence quantitative PCR showed that Fam MOT1 gene was expressed in different degree in roots, stems, leaves, flowers and 10-day-old fruits of strawberry. Different concentrations of sodium molybdate were sprayed on strawberry seedlings. The results showed that the molybdenum concentration in leaves increased significantly with the increase of molybdenum application. The FaMOT1 expression in roots and leaves was significantly induced by the treatment of .2.4 mmol L-1 Na2Mo O4. The highest level of FaMOT1 expression was observed in stems treated with 4.8 mmol L ~ (-1) Na_2MoO_4 for 2 h and 4 h, and the highest FaMOT1 expression level in stems treated with 2.4 mmol L ~ (-1) Na_2MoO_4 at 6 h and 12 h. The nitrate N concentration of leaves treated with 2.4 mmol L ~ (-1) Na_2MoO_4 was significantly lower than that of other treatments. The concentration of ammonia nitrogen was significantly higher than that of other treatments (4.8 mmol L ~ (-1) Na_2MoO_4). The lowest concentration of ammonia nitrogen was found in roots and leaves. The results indicated that the expression of FaMOT1 gene was induced and regulated by molybdenum, and the proper concentration of molybdenum was beneficial to the absorption of nitrate nitrogen, the transformation to ammonia nitrogen and the transformation of ammonia nitrogen to organic nitrogen.
【作者单位】: 作物生物学国家重点实验室山东果蔬优质高效生产协同创新中心山东农业大学园艺科学与工程学院;
【基金】:国家自然科学基金(31672137) 山东省现代农业产业技术体系果品创新团队-栽培与土肥岗(SDAIT-06-01)~~
【分类号】:S668.4
,
本文编号:2166926
[Abstract]:A member of MOT1 family was cloned from 'Zhang Ji' strawberry, and its CDS sequence was found to be consistent with the published genomic sequence of forest strawberry, which was named FaMOT 1. Phylogenetic tree analysis showed that the amino acids encoded by Fa MOT1 were most closely related to apple and peach. The results of fluorescence quantitative PCR showed that Fam MOT1 gene was expressed in different degree in roots, stems, leaves, flowers and 10-day-old fruits of strawberry. Different concentrations of sodium molybdate were sprayed on strawberry seedlings. The results showed that the molybdenum concentration in leaves increased significantly with the increase of molybdenum application. The FaMOT1 expression in roots and leaves was significantly induced by the treatment of .2.4 mmol L-1 Na2Mo O4. The highest level of FaMOT1 expression was observed in stems treated with 4.8 mmol L ~ (-1) Na_2MoO_4 for 2 h and 4 h, and the highest FaMOT1 expression level in stems treated with 2.4 mmol L ~ (-1) Na_2MoO_4 at 6 h and 12 h. The nitrate N concentration of leaves treated with 2.4 mmol L ~ (-1) Na_2MoO_4 was significantly lower than that of other treatments. The concentration of ammonia nitrogen was significantly higher than that of other treatments (4.8 mmol L ~ (-1) Na_2MoO_4). The lowest concentration of ammonia nitrogen was found in roots and leaves. The results indicated that the expression of FaMOT1 gene was induced and regulated by molybdenum, and the proper concentration of molybdenum was beneficial to the absorption of nitrate nitrogen, the transformation to ammonia nitrogen and the transformation of ammonia nitrogen to organic nitrogen.
【作者单位】: 作物生物学国家重点实验室山东果蔬优质高效生产协同创新中心山东农业大学园艺科学与工程学院;
【基金】:国家自然科学基金(31672137) 山东省现代农业产业技术体系果品创新团队-栽培与土肥岗(SDAIT-06-01)~~
【分类号】:S668.4
,
本文编号:2166926
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