耐低氮烟草基因型的筛选及其氮效率类型

发布时间：2018-08-08 11:58

【摘要】：筛选耐低氮及氮高效的烟草基因型是提高氮素利用效率,减少氮污染的一种有效途径。本文采用营养液培养方法,以低氮(0.5 mmol L~(-1))和正常氮(5.0 mmol L~(-1))处理74个烟草基因型幼苗,通过指标统计、因子分析、聚类分析来确定评价指标,并筛选出耐低氮基因型。不同基因型烟草的根系体积、根系生物量、茎叶氮累积量、地上部生物量变异明显,低氮和正常氮处理的变异系数分别为0.37~0.68和0.38~0.64。两种氮水平下的主成分基本相似,并表明茎叶氮累积量和地上部生物量起主要作用。根据评价指标的Heatmap聚类和氮效率综合值散点图分析结果,筛选出15个耐低氮基因型,占供试材料的20.3%,其中8个属于低氮高效正常氮低效型,6个属于低氮低效正常氮低效型,1个属于低氮高效正常氮高效型;还筛选出8个低氮敏感基因型,其中6个属于低氮低效正常氮高效型,2个属于低氮低效正常氮低效型。初步确定14P9为耐低氮且氮高效基因型,中烟100和K394为低氮敏感且氮低效基因型。
[Abstract]:Screening tobacco genotypes with low nitrogen tolerance and high nitrogen efficiency is an effective way to improve nitrogen use efficiency and reduce nitrogen pollution. 74 tobacco genotypic seedlings were treated with low nitrogen (0.5 mmol L ~ (-1) and normal nitrogen (5.0 mmol L ~ (-1). The evaluation indexes were determined by index statistics, factor analysis and cluster analysis, and low nitrogen tolerance genotypes were screened. The root volume, root biomass, stem and leaf nitrogen accumulation and shoot biomass of different genotypes of tobacco varied significantly, and the coefficients of variation of low nitrogen and normal nitrogen treatments were 0.370.68 and 0.38g 0.64, respectively. The principal components of the two nitrogen levels were basically similar, indicating that the nitrogen accumulation in the stems and leaves and the aboveground biomass played a major role. According to the results of Heatmap clustering and nitrogen efficiency scatter map analysis, 15 low nitrogen tolerant genotypes were selected. Among the tested materials, 8 belong to low nitrogen efficiency and normal nitrogen low efficiency type, 6 belong to low nitrogen and low efficiency normal nitrogen low efficiency type, 1 belongs to low nitrogen efficiency and normal nitrogen efficiency type, and 8 low nitrogen sensitive genotypes are screened. Among them, 6 belong to low nitrogen, low efficiency, normal nitrogen efficiency and 2 belong to low nitrogen and low efficiency normal nitrogen and low efficiency type. 14P9 was identified as low nitrogen tolerance and nitrogen efficient genotype, Zhongyan 100 and K394 were low nitrogen sensitive and low nitrogen efficient genotypes.
【作者单位】：江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室/江西省作物生理生态与遗传育种重点实验室;江西省烟叶科学研究所;
【基金】：江西省烟草专卖局科技项目(201401001)资助~~
【分类号】：S572

【参考文献】