棉花PR基因感应大丽轮枝菌病原相关分子模式的表达分析

发布时间：2018-08-16 12:19

【摘要】：由大丽轮枝菌(Verticillium dahliae)侵染所引起的棉花黄萎病,每年给我国农业生产带来巨大的经济损失.同时,棉花与大丽轮枝菌互作过程中发生的免疫反应变化及其机理尚不明确,缺乏棉花免疫反应研究的指示基因.本研究根据已报道的植物病程相关基因(pathogenesis-related gene,PR基因)序列,搜索陆地棉(Gossypium hirsutum)基因组数据库,获得14个潜在的PR基因家族(GhPR).为了明确GhPR在植物免疫反应过程中的表达情况,分别利用两种已报道的病原相关分子模式(PAMP)肽段flg22和nlp20,以及3个来自大丽轮枝菌、与nlp20(nlp20~(Pp))同源的肽段nlp20~(Vd2),nlp23~(Vd3),以及nlp23~(Vd4),对无菌棉花根部进行处理,检测GhPR基因在不同处理时间段的表达情况.结果显示,在flg22,nlp20~(Pp),nlp20~(Vd2),nlp23~(Vd3)和nlp23~(Vd4)处理下,GhPR基因普遍上调表达,且在处理后3 h达到最高值;在处理后12 h内,nlp20~(Vd2)比其他肽段更为有效地诱导GhPR基因上调表达;尽管nlp23~(Vd3)和nlp23~(Vd4)所在的全长蛋白VdNLP3和VdNLP4不能诱导棉花叶片的坏死反应,但是这两个肽段却能够引起GhPR基因的上调表达.因此,本研究为棉花-大丽轮枝菌的互作的研究提供了免疫反应指示基因GhPR,以及来自大丽轮枝菌的免疫原性肽段nlp20~(Vd2),nlp23~(Vd3),以及nlp23~(Vd4).
[Abstract]:Verticillium dahliae caused by (Verticillium dahliae) infection of Verticillium dahliensis brings huge economic loss to agricultural production in China every year. At the same time, the changes and mechanism of the immune reaction in cotton and the interaction between cotton and Verticillium dahliensis were not clear, and the indicative genes for the study of the immune response in cotton were not clear. Based on the reported sequence of plant pathogenesis-related gene (pathogenesis-related gene), the (Gossypium hirsutum) genomic database of Upland cotton (Gossypium hirsutum L.) was searched and 14 potential PR gene families (GhPR). Were obtained in this study. In order to clarify the expression of GhPR in plant immune response, two reported pathogen-associated molecular models, (PAMP) peptide flg22 and nlp20, were used, respectively, and three strains were isolated from Verticillium dahliensis. Nlp20 ~ (Vd2) nlp23 ~ (Vd3) and nlp23 ~ (Vd4) homologous to nlp20 (nlp20 ~ (Pp) were used to detect the expression of GhPR gene in aseptic cotton roots. The results showed that nlp20 ~ (Pp) nlp20 ~ (Vd2) nlp23 ~ (Vd3) and nlp23 ~ (Vd4) generally upregulated the expression of GhPR gene, and reached the highest level at 3 h after treatment, and nlp20 ~ (Vd2) could induce the up-regulation of GhPR gene more effectively than other peptides within 12 h after treatment. Although VdNLP3 and VdNLP4, the full-length proteins of nlp23 ~ (Vd3) and nlp23 ~ (Vd4), could not induce the necrotic response in cotton leaves, these two peptides could induce the up-regulation of GhPR gene expression. Therefore, this study provided the immunoreactive indicator gene GhPRand the immunogenicity peptide nlp20 ~ (Vd2) nlp23 ~ (Vd3) and nlp23 ~ (Vd4) for the study of the interaction between cotton and Verticillium dahliensis.
【作者单位】：中国科学院微生物研究所植物基因组学国家重点实验室;中国科学院大学生命科学学院;石河子大学农学院新疆绿洲农业病虫害治理与植保资源利用重点实验室;德国图宾根大学植物分子生物学中心;
【基金】：中国科学院战略性先导科技专项(批准号:XDB11040500) 国家自然科学基金(批准号:31500119,31600124)资助
【分类号】：S435.62

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