植物中3-磷酸甘油脱氢酶基因家族的物种特异性进化分析
发布时间：2018-08-17 16:39
【摘要】：3-磷酸甘油脱氢酶(GPDH)是一种在磷酸甘油酯的代谢过程中起重要调节作用的关键酶,研究植物中3-磷酸甘油脱氢酶基因家族的进化历史及其多样性对进一步探索其作用机制具有重要意义.以20个已报道的GPDH基因作为查询序列,与19种代表性的植物物种的基因组分别进行BLASTP和t BLASTN比对以及隐马尔科夫模型搜索,获得基因家族新成员,构建系统发育树,并对其进行基因结构、蛋白模体、功能差异性以及适应性进化分析.预测得到了75个新的GPDH基因家族成员,系统发育分析表明,植物中的GPDH基因属于3个单系类群(命名为族群I、族群II和族群III),并且在进化过程中主要分为两个进化枝,说明GPDH基因家族源自两个GPDH祖先基因;功能差异性分析表明,氨基酸特异性位点的选择性约束作用于不同族群的GPDH基因上,并且导致了不同族群多样化之后的特异性进化,同时,在族群I/III和族群II/III中也发现了Type-II功能差异性,表明氨基酸的理化性质也发生了本质的改变;适应性进化分析以及ka/ks比值分析表明,GPDH基因家族在物种特异性复制之后的进化过程中受到了纯化选择作用.因此,GPDH基因家族在其进化过程中受到不同的选择压力而出现了不同的进化方向,形成了多样化的族群.此外,对该基因家族进行的系统的分子进化探索为植物中GPDH基因家族的进一步生物化学和遗传方面的研究以及基因组分析提供了数据基础,在GPDH基因家族分析中的结果与发现也为研究其他基因家族提供了有力的帮助.
[Abstract]:Glycerol 3-phosphate dehydrogenase (GPDH) is a key enzyme that plays an important role in the metabolism of glycerol phosphate. It is important to study the evolutionary history and diversity of glycerol 3-phosphate dehydrogenase gene family in plants. Using 20 reported GPDH genes as query sequences, the genome of 19 representative plant species was compared with BLASTP and t BLASTN and hidden Markov model search. New members of gene family were obtained and phylogenetic tree was constructed. Gene structure, protein motifs, functional differences and adaptive evolution were analyzed. Phylogenetic analysis showed that the GPDH gene in plants belonged to three monoclines (population I, population II and population III), and they were mainly divided into two evolutionary branches during evolution. The functional difference analysis showed that the selective restriction of amino acid specific sites acted on the GPDH gene of different populations and led to the specific evolution of different populations after diversification. At the same time, the functional differences of Type-II were also found in I/III and II/III, indicating that the physical and chemical properties of amino acids had also changed substantially. Adaptive evolution analysis and ka/ks ratio analysis showed that the GPDH gene family was purified and selected during the evolution of species specific replication. As a result, the GPDH gene family is under different selection pressures in its evolution process, resulting in different evolution directions, forming a diverse population. In addition, the systematic molecular evolution of the GPDH gene family provides a data basis for further biochemical and genetic studies and genomic analysis of the GPDH gene family in plants. The results and findings in GPDH gene family analysis also provide powerful help for the study of other gene families.
【作者单位】： 四川大学生命科学学院微生物与代谢工程四川省重点实验室;
【基金】：国家自然科学基金项目(31272659,31171447) “十二五”支撑计划项目(2013BAD10B01,2014BAD02B02) 四川省科技厅项目(2013GZ0058,2014GZX0005,2014JCPT005)资助~~
【分类号】：Q943.2
，
本文编号：2188257
[Abstract]:Glycerol 3-phosphate dehydrogenase (GPDH) is a key enzyme that plays an important role in the metabolism of glycerol phosphate. It is important to study the evolutionary history and diversity of glycerol 3-phosphate dehydrogenase gene family in plants. Using 20 reported GPDH genes as query sequences, the genome of 19 representative plant species was compared with BLASTP and t BLASTN and hidden Markov model search. New members of gene family were obtained and phylogenetic tree was constructed. Gene structure, protein motifs, functional differences and adaptive evolution were analyzed. Phylogenetic analysis showed that the GPDH gene in plants belonged to three monoclines (population I, population II and population III), and they were mainly divided into two evolutionary branches during evolution. The functional difference analysis showed that the selective restriction of amino acid specific sites acted on the GPDH gene of different populations and led to the specific evolution of different populations after diversification. At the same time, the functional differences of Type-II were also found in I/III and II/III, indicating that the physical and chemical properties of amino acids had also changed substantially. Adaptive evolution analysis and ka/ks ratio analysis showed that the GPDH gene family was purified and selected during the evolution of species specific replication. As a result, the GPDH gene family is under different selection pressures in its evolution process, resulting in different evolution directions, forming a diverse population. In addition, the systematic molecular evolution of the GPDH gene family provides a data basis for further biochemical and genetic studies and genomic analysis of the GPDH gene family in plants. The results and findings in GPDH gene family analysis also provide powerful help for the study of other gene families.
【作者单位】： 四川大学生命科学学院微生物与代谢工程四川省重点实验室;
【基金】：国家自然科学基金项目(31272659,31171447) “十二五”支撑计划项目(2013BAD10B01,2014BAD02B02) 四川省科技厅项目(2013GZ0058,2014GZX0005,2014JCPT005)资助~~
【分类号】：Q943.2
，
本文编号：2188257
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