转苋色藜NDR1基因水稻的获得及对白叶枯病抗性的初步研究

发布时间：2018-08-19 09:26

【摘要】：NDR1(non-race-specific disease resistance)基因能介导植物广谱抗病,在植物的防御反应中发挥重要作用。以特色抗病植物苋色藜为材料,克隆并鉴定了NDR1的同源基因Ca NDR1a和Ca NDR1b。在此基础上,利用该基因分别构建植物表达载体,农杆菌介导转化法获得转基因水稻,经PCR和Southern杂交证明外源基因已整合到水稻基因组中。选取部分株系进行水稻白叶枯病抗性鉴定,记录接种后3 d、5 d、10 d的叶片病斑长度,结果表明:转Ca NDR1a、Ca NDR1b基因水稻对白叶枯病具有一定的抗性,可延迟发病。外源基因在转基因水稻植株中均有较高水平表达,但抗病性较好株系Ca NDR1基因的表达量并非最高。农艺性状调查结果显示多数转基因植株株高较对照组矮,但结实率、千粒重等农艺性状与对照组相比并无显著差异。
[Abstract]:NDR1 (non-race-specific disease resistance) gene) can mediate broad spectrum disease resistance and play an important role in plant defense response. The homologous genes Ca NDR1a and Ca NDR1b of NDR1 were cloned and identified from Chenopodium amaranthus. On this basis, the plant expression vectors were constructed with the gene, and the transgenic rice was obtained by Agrobacterium tumefaciens-mediated transformation. PCR and Southern hybridization showed that the exogenous gene had been integrated into the rice genome. Some lines were selected to identify the resistance to bacterial blight, and the length of leaf spot was recorded at 3 days and 5 days after inoculation. The results showed that transgenic rice with Ca NDR1a and Ca NDR1b gene had certain resistance to bacterial blight, which could delay the onset of bacterial blight. There was a high level of expression of exogenous genes in transgenic rice plants, but the expression of Ca NDR1 gene was not the highest in the lines with better resistance to disease. The results of agronomic traits investigation showed that the height of most transgenic plants was shorter than that of the control group, but there was no significant difference in agronomic characters such as seed setting rate and 1000-grain weight between the transgenic plants and the control group.
【作者单位】：中国农业科学院生物技术研究所;
【基金】：国家转基因生物新品种培育重大专项(2014ZX08001-002)资助
【分类号】：S435.11

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