巴西橡胶树α-微管蛋白HbTUA2基因的克

发布时间：2018-08-20 19:17

【摘要】：微管蛋白是微管的重要组成部分,参与维持细胞骨架的稳定及延伸。本研究采用RT-PCR和RACE技术从巴西橡胶树(Hevea brasiliensis)中克隆到一个α-微管蛋白基因,命名为Hb TUA2(Gen Bank登录号:KX809698)。该基因c DNA全长1 940bp,其中开放阅读框1 353 bp,编码含450个氨基酸残基的蛋白质序列。Hb TUA2与其他植物TUA蛋白高度同源,具有TUA蛋白家族保守的GTP结合位点和转录后调控片段。基因结构分析表明,Hb TUA2具有4个外显子和3个内含子,内含子外显子剪切符合"GT-AG"规则。荧光定量PCR分析表明,Hb TUA2在巴西橡胶树叶片、雌花、雄花、茎尖、树皮及胶乳等组织中均有表达,在雌花中表达量最高,其次是茎尖和雄花,在叶片、树皮和胶乳中表达量较低。此外,Hb TUA2的表达还受高盐、干旱、低温、过氧化氢(H_2O_2)、乙烯利和茉莉酸甲酯(Me JA)调控,表明Hb TUA2可能参与巴西橡胶树乙烯和茉莉酸信号途径及逆境胁迫应答过程。
[Abstract]:Tubulin, an important component of microtubules, is involved in maintaining the stability and extension of cytoskeleton. In this study, a 伪 -tubulin gene was cloned from (Hevea brasiliensis) of Hevea brasiliensis by RT-PCR and RACE techniques and named as HB TUA2 (Gen Bank accession number: KX809698). The full length of c DNA is 1 940 BP, in which open reading frame 1 353 BP, encoding 450 amino acid residues of protein sequence. HB TUA2 and other plant TUA protein highly homologous, with TUA protein family conserved GTP binding sites and post-transcriptional regulatory fragments. The gene structure analysis showed that Hb TUA2 had 4 exons and 3 introns, and the intron exon shearing was in accordance with the "GT-AG" rule. Fluorescence quantitative PCR analysis showed that Hb TUA2 was expressed in leaves, female flowers, male flowers, stem tips, bark and latex tissues of Hevea brasiliensis, and was the highest in female flowers, followed by stem tips and male flowers, and in leaves. The expression level was lower in bark and latex. In addition, the expression of HB TUA2 was regulated by high salt, drought, low temperature, hydrogen peroxide (H_2O_2), ethephon and methyl jasmonate (Me JA), suggesting that HB TUA2 may be involved in ethylene and jasmonic acid signaling pathway and stress response process in Hevea brasiliensis.
【作者单位】：中国热带农业科学院橡胶研究所农业部橡胶树生物学与遗传资源利用重点实验室;
【基金】：国家自然科学基金(31570684、31270651和31200514) 中国热带农业科学院橡胶研究所基本科研业务费专项(1630022015003)~~
【分类号】：S794.1

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