IFN-α对猪黄体细胞增殖及VEGF基因表达的影响

发布时间：2018-08-22 08:05

【摘要】：为了探讨α干扰素(IFN-α)对体外培养猪黄体细胞增殖及VEGF基因表达的影响,试验分离猪卵巢中的黄体组织进行消化处理,制成细胞悬液,体外培养猪黄体细胞,采用MTT比色法测定猪黄体细胞的增殖,ELISA法测定培养液中孕酮的浓度;Real time PCR和Western-blot检测血管内皮生长因子(VEGF)基因表达。结果表明:IFN-α对猪黄体细胞增殖的抑制作用随着浓度升高而增强。在IFN-α浓度低于100 IU/m L时,对猪培养黄体细胞分泌孕酮呈现抑制作用;当IFN-α浓度高于100 IU/m L时,对猪培养黄体细胞分泌孕酮呈现促进作用。IFN-α下调猪黄体细胞中VEGF mRNA和VEGF蛋白表达。说明IFN-α抑制黄体细胞增殖并具有浓度依赖性,对猪黄体细胞中孕酮的分泌没有影响,下调猪黄体细胞中VEGF基因的表达。
[Abstract]:In order to investigate the effect of interferon 伪 (IFN- 伪) on the proliferation and VEGF gene expression of porcine luteal cells in vitro, porcine luteal cells were isolated from porcine ovaries and digested to form cell suspensions, and porcine luteal cells were cultured in vitro. MTT colorimetric assay was used to detect the proliferation of porcine luteal cells. Real time PCR of progesterone and (VEGF) gene expression of vascular endothelial growth factor (VEGF) in culture medium were determined by Elisa. The results showed that the inhibitory effect of WIFN- 伪 on the proliferation of porcine luteal cells increased with the increase of concentration. When the concentration of IFN- 伪 was below 100 IU/m L, the secretion of progesterone in porcine luteal cells was inhibited, and when IFN- 伪 concentration was higher than 100 IU/m L, the secretion of progesterone in porcine luteal cells was promoted. IFN- 伪 down-regulated the expression of VEGF mRNA and VEGF in porcine luteal cells. These results indicated that IFN- 伪 inhibited the proliferation of luteal cells in a concentration-dependent manner and had no effect on the secretion of progesterone in porcine luteal cells. IFN- 伪 down-regulated the expression of VEGF gene in porcine luteal cells.
【作者单位】：黑龙江省家畜遗传资源保护中心;东北农业大学动物科学技术学院;
【基金】：黑龙江省教育厅规划课题(GBC1214020) 哈尔滨市科技创新人才项目(2016RAQXJ083)
【分类号】：S828

【参考文献】