基于RNA-seq的套袋对‘天源红’猕猴桃果实花色苷合成关键结构基因表达的影响

发布时间：2018-08-24 11:43

【摘要】：【目的】探讨套袋对红肉猕猴桃果实生长发育过程中不同部位花色苷合成关键结构基因表达的影响,为解析光照对花色苷合成分子机制的影响提供依据。【方法】以全红型软枣猕猴桃‘天源红’为试材并对其进行套袋处理,在RNA-seq的基础上,筛选候选基因,利用实时荧光定量PCR对这些候选基因在‘天源红’盛花后7个时期的果肉样品中的表达量进行相对定量。根据荧光定量结果和聚类分析,最终确定关键候选基因,利用实时荧光定量PCR对关键候选基因在‘天源红’不同处理、不同时期、不同果实部位中的表达量进行相对定量分析,并对关键候选基因的表达量和花色苷含量进行相关性分析。【结果】12个候选基因在盛花后7个时期中的表达具有一定的规律,且各自的表达规律不尽相同。在果肉颜色明显变为红色的盛花后110 d,F3H、LDOX和F3GT的表达量较其他时期高。聚类分析结果显示,LDOX和F3GT分别聚为一类,且与其他的基因明显分开。LDOX在处理1和处理2的果肉以及处理3的果心中的表达量与花色苷含量呈极显著相关,而F3GT在处理1的果肉和处理3的果心中的表达量与花色苷含量呈极显著相关。【结论】LDOX和F3GT可能是‘天源红’果实花色苷生物合成途径中的关键结构基因。套袋抑制LDOX在‘天源红’外果皮、果肉中的表达,而一直套袋促进其在果心中的表达。套袋抑制了F3GT在果肉中的表达,而对果心中F3GT的表达无明显影响。
[Abstract]:[Objective] To investigate the effect of Bagging on the expression of key genes involved in anthocyanin biosynthesis in different parts of red Kiwifruit during its growth and development, and to provide basis for the analysis of the molecular mechanism of anthocyanin biosynthesis under illumination. On this basis, candidate genes were screened, and the expression of these candidate genes in pulp samples at 7 stages after flowering was quantified by real-time fluorescence quantitative PCR. According to the results of fluorescence quantitative analysis and cluster analysis, the key candidate genes were finally identified, and the key candidate genes were identified by real-time fluorescence quantitative PCR. The relative quantitative analysis of the expression of the key candidate genes and the content of anthocyanin were carried out in different fruits of different treatments and different stages. The results of cluster analysis showed that LDOX and F3GT were clustered into one group and were distinctly separated from other genes. The expression of LDOX in the flesh of treatment 1 and treatment 2 and in the heart of treatment 3 was significantly correlated with anthocyanin content, whereas F3GT was present in the flesh of treatment 1 and treatment 2. [Conclusion] LDOX and F3GT may be the key structural genes in anthocyanin biosynthesis pathway of'Tianyuan Red'fruit. Bagging inhibits the expression of LDOX in the exocarp and pulp of'Tianyuan Red', and bagging promotes the expression of LDOX in the core of'Tianyuan Red'. Bagging inhibited the expression of F3GT in pulp, but had no significant effect on the expression of F3GT in fruit.
【作者单位】：中国农业科学院郑州果树研究所·中国农业科学院果树生长发育与品质控制重点开放实验室;
【基金】：国家自然科学基金(31401825) 中国农业科学院科技创新工程专项(CAAS-ASTIP-2016-ZFRI) 河南省大宗水果产业技术体系(S2014-11)
【分类号】：S663.4

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