扶正解毒化瘀方对CRF营养不良大鼠IL-6、Leptin及Ob基因mRNA表达的影响

发布时间：2018-08-24 12:52

【摘要】：目的:本实验是探讨慢性肾衰竭营养不良大鼠的发病机理,并观察扶正解毒化瘀方对IL-6、Leptin及Ob基因m RNA表达的调控机制,为今后寻找中医药方法治疗慢性肾衰竭合并症的患者提供实验依据,同时为丰富慢性肾衰竭的病机与治法理论提供新的思路和方法。方法:将中医药的传统理论和现代生物分子学相结合,提出(本虚标实)“脾肾两虚,瘀血内阻、浊毒内停”的发病病机,以补脾益肾,活血化瘀、解毒泄浊”为治疗大法,精选药物,合理组方,制成扶正解毒化瘀方。将40只SD大鼠随机分为对照组、模型组、中药组(扶正解毒化瘀方)和西药组(益比奥),以腺嘌呤饲养建立慢性肾衰大鼠模型,检测各组大鼠生化指标(BUN、Scr、ALB、Hb),采用酶联免疫吸附剂测定(ELISA)法检测肾脏组织中Leptin、IL-6的水平,RT-PCR技术检测Ob基因m RNA表达的水平。结果:1.扶正解毒化瘀方组大鼠用药后BUN、Scr、Leptin、IL-6水平较模型组明显下降(P0.01),大鼠体重增加,Alb、Hb提高,贫血营养不良程度改善;且中药组Scr降幅度较西药组大(P0.05)。2.中药组与空白对照组、模型组对比CRF大鼠肾脏组织Leptin的Ob基因m RNA的表达在用药后明显降低(P0.05)。结论:1.脾肾两虚,瘀血内阻、浊毒内停是导致CRF营养不良的主要病机,以补脾益肾,活血化瘀、解毒泄浊”为其治疗大法,有效保护CRF大鼠肾功能,改善CRF大鼠营养不良的状态。2.IL-6的水平升高刺激Ob基因,Ob基因m RNA的表达水平与Leptin有直接调控作用。3.慢性肾衰竭明显存在炎症因子IL-6和高Leptin,且IL-6和Leptin对慢性肾衰竭营养不良状态出现有密切的关系,用扶正解毒化瘀方后可能通过调节Ob基因的活性而改善营养不良状态。
[Abstract]:Objective: to investigate the pathogenesis of chronic renal failure (CRF) dystrophy in rats, and to observe the regulatory mechanism of Fuzheng jiedu Huayu recipe (Fuzheng jiedu Huayu recipe) on the expression of IL-6,Leptin and Ob gene m RNA in rats. It provides experimental basis for the treatment of patients with chronic renal failure in the future, and provides new ideas and methods for enriching the pathogenesis and treatment theory of chronic renal failure. Methods: combining the traditional theory of traditional Chinese medicine with modern biomolecules, the pathogenesis of "deficiency of spleen and kidney, internal obstruction of blood stasis, stagnation of turbid toxin" was put forward, which was treated by replenishing the spleen and tonifying the kidney, activating blood circulation and removing blood stasis, and detoxifying turbid blood. Selected drugs, reasonable formulation, made of Fuzheng jiedu Huayu prescription. Forty SD rats were randomly divided into control group, model group, traditional Chinese medicine group (Fuzheng jiedu Huayu prescription) and western medicine group (Yibiao). The biochemical index (BUN,Scr,ALB,Hb) of rats in each group was detected, and the level of Leptin,IL-6 in kidney tissue was detected by (ELISA) method with enzyme-linked immunosorbent assay (Elisa). The expression of Ob gene m RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). The result is 1: 1. The level of BUN,Scr,Leptin,IL-6 in Fuzheng jiedu Huayu prescription group was significantly lower than that in the model group (P0.01), the weight gain of rats was increased and the degree of anemia and malnutrition was improved, and the decrease rate of Scr in the traditional Chinese medicine group was larger than that in the western medicine group (P0.05). In Chinese medicine group and blank control group, the expression of Ob gene m RNA in renal tissue of CRF rats was significantly decreased after treatment (P0.05). Conclusion 1. Deficiency of spleen and kidney, internal obstruction of blood stasis and stagnation of turbid toxin are the main pathogenesis leading to CRF malnutrition. The main treatment method is tonifying spleen and tonifying kidney, activating blood circulation and removing blood stasis, detoxifying turbid, and effectively protecting renal function of CRF rats. To improve the status of dystrophy in CRF rats. 2. The elevation of IL-6 stimulated the expression of m RNA of Ob gene, which was directly regulated by Leptin. The inflammatory factors IL-6 and high Leptin, were obviously existed in chronic renal failure and IL-6 and Leptin were closely related to the occurrence of chronic renal failure malnutrition. After using Fuzheng jiedu Huayu recipe to improve the status of malnutrition by regulating the activity of Ob gene.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R285.5

【相似文献】