农杆菌T-DNA传递相关基因研究进展

发布时间：2018-08-26 15:31

【摘要】：农杆菌已广泛应用于单、双子叶植物的遗传转化。T-DNA的传递是农杆菌介导转化的分子基础。T-DNA的传递是一个复杂的过程,与Ti质粒毒性区基因(Vir)、农杆菌染色体上毒性相关基因(chv)有关,另外植物体内部分基因也参与T-DNA的传递。T-DNA传递时在VirD1-VirD2蛋白的作用下形成T链,进一步形成T复合物,经农杆菌四型分泌系统(Type IV secretion system,T4SS)穿过细菌和植物细胞膜,运输到植物细胞胞质。进入植物细胞的T复合物在植物相关蛋白的作用下经核运输和T链的整合,最终整合到植物基因组。本研究从农杆菌对植物细胞的识别和附着、农杆菌对植物信号的感知和Vir基因的活化、T链的形成、T-复合物的运输、T链进入植物细胞后的核运输和T链的整合机制及相关基因作了详细综述,探讨了农杆菌转化机制研究中的问题,对今后农杆菌转化机制研究做了展望。
[Abstract]:Agrobacterium tumefaciens has been widely used in monocotyledonous plants. The transmission of T-DNA is the molecular basis of Agrobacterium tumefaciens transformation. T-DNA transfer is a complex process. It is related to the toxic gene (chv) on the chromosome of Agrobacterium tumefaciens (Vir), which is the toxic region gene of Ti plasmid. In addition, some genes in plant also participate in the transmission of T-DNA. T-DNA is involved in the transmission of VirD1-VirD2 protein to form T chain and further form T complex. Through Agrobacterium tumefaciens type IV secretory system (Type IV secretion system,T4SS) transports through bacteria and plant cell membranes to plant cytoplasm. The T complex which enters the plant cells is transported through the nucleus and the T chain is integrated into the plant genome under the action of plant related proteins. In this study, the identification and attachment of plant cells by Agrobacterium tumefaciens were studied. Agrobacterium tumefaciens' perception of plant signal and the formation of T chain activated by Vir gene were reviewed in detail. The nuclear transport and T chain integration mechanism and related genes of T- complex transporter T chain into plant cells were reviewed in detail. The problems in the study of Agrobacterium tumefaciens transformation mechanism were discussed, and the future research of Agrobacterium tumefaciens transformation mechanism was prospected.
【作者单位】：国家谷子改良中心河北省农林科学院谷子研究所河北省杂粮研究实验室;
【基金】：河北省科技厅项目(16227508D-8) 国家谷子糜子产业技术体系项目(CARS-07-13.5-A3) 河北省农林科学院博士基金项目(F17E02);河北省农林科学院青年基金项目(A2015030102) 河北省自然科学基金项目(C2017301085) 河北省科技计划项目(16227508D-8;14236314D) 北京市科委课题(Z161100000916003)共同资助
【分类号】：Q943.2

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