江苏地区对虾3种病毒病的流行病学调查及5株IHHNV的编码区基因序列分析
发布时间:2018-08-30 16:33
【摘要】:对虾白斑综合征病毒(WSSV)、桃拉综合征病毒(TSV)、传染性皮下及造血组织坏死病毒(IHHNV)是威胁对虾养殖的重要病毒。为了调查这3种病在江苏境内的流行情况,根据世界动物卫生组织(OIE)推荐的《水生动物疾病诊断手册》的PCR检测法对2015年5月—2016年5月采集的1436尾对虾样品进行3种疾病的流行病学调查。结果显示WSSV阳性率为17.20%,TSV阳性率为0%,IHHNV阳性率为39.48%。对江苏不同地区分离出的5株IHHNV进行基因序列分析,数据显示这5个地区的毒株均属于Ⅰ型感染株,与韩国株的进化关系较为接近。本研究通过调查以上3种病毒病在江苏境内的流行情况,并进行序列分析,发现江苏不同地区的凡纳滨对虾IHHNV感染均为Ⅰ型,研究结果对养殖虾的疾病防控有重要参考价值。
[Abstract]:White spot syndrome virus (WSSV),) and hematopoietic necrosis virus (IHHNV) of (WSSV), peach syndrome virus (TSV),) are important viruses threatening shrimp culture. In order to investigate the prevalence of these three diseases in Jiangsu, 1436 shrimp samples collected from May 2015 to May 2016 were investigated by the PCR method recommended by the World Animal Health Organization (OIE) for the diagnosis of aquatic animal diseases. The results showed that the positive rate of WSSV was 17.20%. The positive rate of WSSV was 39.48%. Five strains of IHHNV isolated from different regions of Jiangsu Province were sequenced. The data showed that all of the five strains belonged to type 鈪,
本文编号:2213638
[Abstract]:White spot syndrome virus (WSSV),) and hematopoietic necrosis virus (IHHNV) of (WSSV), peach syndrome virus (TSV),) are important viruses threatening shrimp culture. In order to investigate the prevalence of these three diseases in Jiangsu, 1436 shrimp samples collected from May 2015 to May 2016 were investigated by the PCR method recommended by the World Animal Health Organization (OIE) for the diagnosis of aquatic animal diseases. The results showed that the positive rate of WSSV was 17.20%. The positive rate of WSSV was 39.48%. Five strains of IHHNV isolated from different regions of Jiangsu Province were sequenced. The data showed that all of the five strains belonged to type 鈪,
本文编号:2213638
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