基于COI基因序列的泥蚶遗传多样性和种群结构分析

发布时间：2018-09-01 10:13

【摘要】：为了研究泥蚶不同地理群体的遗传多样性和它们的系统进化关系,以采自来自7个地理群体的150个泥蚶个体为材料,对其线粒体细胞色素氧化酶亚基I(COI)的部分序列进行PCR扩增和序列比对,分析泥蚶群体的遗传多样性;计算泥蚶不同地理群体间的遗传距离及遗传分化系数,基于泥蚶群体平均遗传距离构建UPGMA聚类树;以毛蚶Anadara sativa为外群,用MEGA6.0软件中的ML法构建了泥蚶单倍型之间的系统进化树以及用Network4.6软件分析了所有单倍型的中介网络图。结果表明,泥蚶线粒体COI的基因片段的PCR产物大小约为509 bp。在目的序列片段中检测到了14个多态性位点,包括6个简约信息位点和8个单变异多态性位点,组成16个单倍型。群体中的核苷酸多态性和单倍型多态性均处于较低的水平,日本、海南、韩国三个群体的遗传变异程度相对较高。7个群体间的遗传距离在0.0002~0.0022之间,种群遗传分化系数(FST)值在-0.01646~0.34422之间。通过构建UPGMA聚类树,发现广西、江苏、山东三个群体聚成一个小支,之后依次和浙江、海南、韩国、日本聚在一起,但总体而言泥蚶的不同地理群体之间并没有形成明显的遗传分化。ML系统进化树和中介网络图表明在16个单倍型中,各单倍型之间未形成与其地理群体空间分布相对应的聚类单元,也不存在明显的进化关系。
[Abstract]:In order to study the genetic diversity and phylogenetic relationship among different geographic populations, 150 individuals from 7 geographic groups were used as materials. The partial sequences of mitochondrial cytochrome oxidase subunit (I (COI) were amplified by PCR and compared with each other to analyze the genetic diversity of clams, and to calculate the genetic distance and genetic differentiation coefficient among different populations. The UPGMA clustering tree was constructed based on the average genetic distance of the population, the phylogenetic tree between haplotypes was constructed by ML method in MEGA6.0 software, and the network diagram of all haplotypes was analyzed by Network4.6 software. The results showed that the PCR product of mitochondrial COI was about 509 bp.. Fourteen polymorphic loci were detected in the target sequence, including 6 parsimonic information loci and 8 single mutation polymorphic loci, forming 16 haplotypes. The nucleotide polymorphism and haplotype polymorphism in the population were at a lower level, and the genetic variation was relatively high in Japan, Hainan and South Korea. The genetic distance between the seven populations was between 0.0002 and 0.0022. The genetic differentiation coefficient (FST) of the population was between -0.01646 and 0.34422. By constructing the UPGMA cluster tree, we find that Guangxi, Jiangsu and Shandong are grouped into a small branch, and then together with Zhejiang, Hainan, South Korea and Japan in turn. But in general, there was no significant genetic differentiation between different geographic populations of clams. ML phylogenetic tree and intermediary network showed that in 16 haplotypes, There is no cluster unit corresponding to the spatial distribution of the geographic population among the haplotypes, and there is no obvious evolutionary relationship between the haplotypes.
【作者单位】：浙江省海洋水产养殖研究所;浙江省近岸水域生物资源开发与保护重点实验室;中国水产科学研究院海洋贝类工程技术研究中心;温州医科大学检验医学院生命科学学院;
【基金】：国家高技术研究发展计划(2012AA10A410-1) 浙江省重大科技专项(2012C12907-4) 浙江省科技计划项目(2013F50016) 温州市种子种苗科技创新专项(N20120017)
【分类号】：S917.4

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