喜盐鸢尾花色形成关键基因的克隆及表达分析

发布时间：2018-09-02 06:19

【摘要】：喜盐鸢尾(Iris halophila Pall.)及其变种蓝花喜盐鸢尾(I.halophila Pall.var.sogdiana(Bung)Grubov)因耐盐碱及其多种花色而具有盐碱地园艺开发价值。本文根据喜盐鸢尾内轮花被转录组测序结果,利用基因特异性引物从这2种植物中分别克隆了编码查尔酮合成酶(CHS)、查尔酮异构酶(CHI)、类黄酮-3',5'-羟基化酶类(F3'5'H-like)等基因的部分片段,并对它们在内轮花被中的表达水平进行实时定量PCR分析。序列分析结果确认在喜盐鸢尾中所克隆的CHS(311 bp)、CHI(457 bp)、F3'5'H-like(496 bp)3个基因(部分)未见文献报道与NCBI等数据库记录。其中F3'5'H-like基因与经典的属于细胞色素P450CYP75A亚家族的F3'5'H不同,而与万带兰的F3'5'H-like同属于CYP76AB亚家族,为一类新的蓝花相关基因。实时定量PCR表达分析结果表明,与黄花的喜盐鸢尾相比,蓝花喜盐鸢尾中CHS与F3'5'H-like显著上调表达,可能是其花色不同于喜盐鸢尾的主要原因。
[Abstract]:(Iris halophila Pall.) I.halophila Pall.var.sogdiana (Bung) Grubov) has the value of horticultural development in saline-alkali soil because of its saline-alkali tolerance and its various flower colors. Based on the sequence of the internal perianth transcriptome of Iris hyalurium, some fragments of the genes encoding chalcone synthase, (CHS), (CHI), flavonoid -3ketoisomerase (CHI),) and hydroxy enzymes (F3'5'H-like) were cloned from the two plants by gene specific primers. The expression level of their inner ring perianth was analyzed by real-time quantitative PCR. The results of sequence analysis confirmed that the three genes (part) of CHS (311 bp) Chi (457 bp) F3O5H-like (496 bp) cloned from Iris salina were not recorded in literature and NCBI database. The F3'5'H-like gene is different from the classical F3O5H which belongs to the cytochrome P450CYP75A subfamily, but belongs to the CYP76AB subfamily with the F3'5'H-like of Wanzan orchid, which is a new blue flower related gene. The results of real-time quantitative PCR analysis showed that the expression of CHS and F3'5'H-like was significantly up-regulated in the blue flower salt Iris compared with the salt-loving iris of yellow flowers, which may be the main reason for the difference between the flower color and the salt-loving iris.
【作者单位】：中央民族大学生命与环境科学学院;
【基金】：国家自然科学基金项目(31370356) 中央民族大学一流大学一流学科项目(YLDX01013、2015MDTD08C)
【分类号】：S682.19

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