齐口裂腹鱼白介素-1β基因的克隆及表达谱
发布时间：2018-09-03 19:41
【摘要】：探讨齐口裂腹鱼白介素-1β(Interleukin-1β,IL-1β)基因的基本特点。用RT-PCR方法以齐口裂腹鱼脾脏c DNA作为模板扩增IL-1β基因,用生物信息学软件分析其序列,用RT-PCR方法检测IL-1β基因在齐口裂腹鱼6种组织的表达情况。克隆获得的IL-1β序列长1 252 bp,共编码276个氨基酸。Gen Bank序列号为KU886235。IL-1β蛋白相对分子量为31.25 k D,等电点5.45,预测为亲水性蛋白。二级结构主要结构元件为随机卷曲和延伸链,有4个N-糖基化位点和16个磷酸化位点。NJ法系统进化树显示,齐口裂腹鱼与鲤鱼、鲫鱼的亲缘关系最近。组织表达分析显示,IL-1β基因主要在脾脏和肾脏中表达。这为深入研究鱼IL-1β的生物学功能提供理论依据。
[Abstract]:To study the basic characteristics of interleukin-1 尾 (Interleukin-1 尾) gene in fish. The IL-1 尾 gene was amplified by using c DNA of the spleen of the fish by RT-PCR method. The sequence of IL-1 尾 gene was analyzed by bioinformatics software. The expression of IL-1 尾 gene in 6 kinds of tissues was detected by RT-PCR method. The cloned IL-1 尾 sequence (1 252 bp,) encodes 276 amino acids. Gen Bank sequence number is 31.25 KD, isoelectric point 5.45, predicted to be hydrophilic protein. The main structural elements of the secondary structure were random curl and extension chain. There were 4 N-glycosylation sites and 16 phosphorylation sites. NJ phylogenetic tree showed that the phylogenetic relationship between Carp and Carp, Carassius auratus was the closest. Tissue expression analysis showed that IL-1 尾 gene was mainly expressed in spleen and kidney. This provides a theoretical basis for the further study of the biological function of fish IL-1 尾.
【作者单位】： 西南民族大学青藏高原研究院;西南民族大学生命科学与技术学院;
【基金】：四川省科技支撑项目(2016NZ0044) 西南民族大学研究生学位点建设项目(2016XWD-SO71007)
【分类号】：S917.4;Q78
[Abstract]:To study the basic characteristics of interleukin-1 尾 (Interleukin-1 尾) gene in fish. The IL-1 尾 gene was amplified by using c DNA of the spleen of the fish by RT-PCR method. The sequence of IL-1 尾 gene was analyzed by bioinformatics software. The expression of IL-1 尾 gene in 6 kinds of tissues was detected by RT-PCR method. The cloned IL-1 尾 sequence (1 252 bp,) encodes 276 amino acids. Gen Bank sequence number is 31.25 KD, isoelectric point 5.45, predicted to be hydrophilic protein. The main structural elements of the secondary structure were random curl and extension chain. There were 4 N-glycosylation sites and 16 phosphorylation sites. NJ phylogenetic tree showed that the phylogenetic relationship between Carp and Carp, Carassius auratus was the closest. Tissue expression analysis showed that IL-1 尾 gene was mainly expressed in spleen and kidney. This provides a theoretical basis for the further study of the biological function of fish IL-1 尾.
【作者单位】： 西南民族大学青藏高原研究院;西南民族大学生命科学与技术学院;
【基金】：四川省科技支撑项目(2016NZ0044) 西南民族大学研究生学位点建设项目(2016XWD-SO71007)
【分类号】：S917.4;Q78
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