几种植物花分生组织决定基因APETALA1密码子使用模式比较

发布时间：2018-09-04 12:44

【摘要】：为了揭示花分生组织决定基因AP1(APETALA1)的分子机制以及物种间的进化关系,从GenBank数据库挑选8个不同植物AP1基因编码序列,利用R软件分别计算GC含量、有效密码子数(Effective number of codons,ENC)、同义密码子相对使用度(Relative synonymous codon usage,RSCU)、相对密码子使用偏好性(Relative codon usage bias,RCBS)等参数,分析比较不同植物AP1基因密码子使用模式中密码子偏好性和碱基组成动力学。结果表明,不同植物AP1基因密码子使用受到GC偏好性(GCbias)影响,特别是GC3s;大多数最优密码子偏好使用以A/U结尾,其中不同植物AP1基因中ACA、AUA和CAG均表现为较高的RSCU值,为最优势密码子;芍药(Paeonia lactiflora)和牡丹(Paeonia suffruticosa)AP1基因的密码子使用特点相似,碧桃(Prunus persica)和苹果(Malus×domestica)相似;较低的最优密码子频率Fop值和较高的ENC值暗示了不同植物AP1基因在密码子使用中具有一个较弱的偏好性。
[Abstract]:In order to reveal the molecular mechanism of flower meristem determinant gene AP1 (APETALA1) and the evolutionary relationship between species, eight different plant AP1 gene coding sequences were selected from GenBank database and GC content was calculated by R software. Effective codon number (Effective number of codons,ENC), synonymous codon relative usage degree (Relative synonymous codon usage,RSCU) and relative codon usage preference (Relative codon usage bias,RCBS) were analyzed and compared in different plant AP1 codon usage patterns. The results showed that the codon use of AP1 gene in different plants was influenced by GC preference (GCbias), especially in GC3s; most of the optimal codon preference ended with A / U, in which ACA,AUA and CAG in different plant AP1 genes showed higher RSCU values. The codon usage characteristics of (Paeonia lactiflora) and peony (Paeonia suffruticosa) AP1 genes were similar, while that of (Prunus persica) and Malus 脳 domestica were similar. The lower optimal codon frequency Fop value and higher ENC value suggest that different plant AP1 genes have a weak preference for codon use.
【作者单位】：扬州大学动物科学与技术学院;扬州大学园艺与植物保护学院江苏省作物遗传生理重点实验室;
【基金】：国家自然科学基金项目(31372097,31400592) 江苏省高校自然科学研究重大项目(13KJA210005)
【分类号】：Q943.2

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