沙柳SpsLAZY1a和SpsLAZY1b基因克隆及生物信息学分析

发布时间：2018-09-06 10:46

【摘要】：LAZY1基因对于植物分枝角度、分枝方向具有重要影响。以沙柳为试验材料克隆LAZY1基因,利用生物信息学软件分析LAZY1基因序列、构建进化树,并预测蛋白结构。结果表明,SpsLAZY1a和SpsLAZY1b的CDS全长分别为1 161、1 143bp,分别编码386和380个氨基酸。生物信息学分析表明SpsLAZY1a、SpsLAZY1b2种蛋白均为亲水性蛋白,均无信号肽区域。氨基酸序列具有IGT基因家族典型结构域,并且其C端有LAZY1蛋白的典型EAR结构。系统发育树将18个LAZY1蛋白家族聚为2个大类,SpsLAZY1a和SpsLAZY1b属于其中第1类,均为双子叶植物,并与其他植物具有较高相似性。氨基酸同源结构比对发现这些植物间既有相似同源区域,也存在一定差异区域。单子叶比双子叶植物缺少某些同源区域,草本比木本植物又缺少某些同源区域。LAZY1基因在沙柳进化过程中发生基因重复,本研究由沙柳中成功克隆SpsLAZY1a、SpsLAZY1b基因,隶属IGT基因家族,并初步揭示LAZY基因的系统进化关系。
[Abstract]:LAZY1 gene plays an important role in branching angle and branching direction. The LAZY1 gene was cloned from Salix willow and the sequence of LAZY1 gene was analyzed by bioinformatics software to construct the evolutionary tree and predict the protein structure. The results showed that the CDS length of SpsLAZY1a and SpsLAZY1b was 1161 BP and 380 amino acids, respectively. Bioinformatics analysis showed that all SpsLAZY1a,SpsLAZY1b2 proteins were hydrophilic and had no signal peptide region. The amino acid sequence has the typical domain of IGT gene family, and its C-terminal has the typical EAR structure of LAZY1 protein. In phylogenetic tree, 18 LAZY1 protein families were grouped into two large classes, SpsLAZY1a and SpsLAZY1b, belonging to the first group, both dicotyledonous plants, and highly similar to other plants. The comparison of amino acid homology revealed that there were not only similar homologous regions but also some difference regions among these plants. Monocotyledon lacks some homologous regions than dicotyledonous plants, and herbaceous plants lack some homologous regions. LAZY1 gene repeats during the evolution of Salix willow. In this study, SpsLAZY1a,SpsLAZY1b gene was cloned from Salix wilfordii and belonged to IGT gene family. The phylogenetic relationship of LAZY gene was revealed.
【作者单位】：内蒙古农业大学林学院;
【基金】：国家自然科学基金项目(31360187) 林木遗传育种国家重点实验室(中国林业科学研究院)开放课题(TGB2015001)
【分类号】：S793.9

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