电针干预对吗啡耐受形成中小鼠的生物节律及其相关钟基因Per 1和Per 2表达的影响
发布时间：2018-09-06 12:12
【摘要】：目的观察电针干预对吗啡受形成中小鼠的生物节律及其相关钟基因Per1和Per2表达的影响。方法将小鼠24只,随机分为空白对照组(n=8),耐受模型组(n=8),电针干预组(n=8)。耐受模型组、电针干预组皮下注射盐酸吗啡注射液以建立吗啡耐受模型。电针干预组每日上午注射吗啡后开始用电针行双侧"肾俞"穴(BL 23)电针治疗。各组在第1、3、5、7d行小鼠光热甩尾潜伏期测定。第7 d在测定痛阈后处死小鼠取视交叉上核(Suprachiasmatic nucleus,SCN)组织,用荧光定量PCR(RT-PCR)方法检测小鼠SCN中Per1、Per2基因的表达变化。结果 (1)痛阈:耐受模型组和电针干预组在第1、3、5d注射吗啡后,测得痛阈值明显升高,与其初痛阈相比差异明显;电针干预组第7d痛阈与初痛阈相比有差异。(2)自发性昼夜活动节律:耐受模型组喝电针干预组与空白对照组相比,小鼠周期、总活动期、振幅结果均有差异:空白对照组与耐受模型组和电针干预组相比小鼠实验前后振幅差值有差异,耐受模型组与电针治疗组相比具有差异。(3)Per1、Per2基因在SCN中的表达水平:与空白对照组相比,耐受模型组的相对表达量下调;电针干预组与耐受模型组相比较结果又明显差异。结论电针干预组方法可以通过上调吗啡耐受模型小鼠的节律相关基因Per1、Per2的表达量来减缓吗啡耐受小鼠痛阈值的下降趋势,延缓耐受的形成并纠正振幅降低为表现的生物节律紊乱。
[Abstract]:Objective to observe the effects of electroacupuncture intervention on the biological rhythm and related clock gene Per1 and Per2 expression in morphine-induced mice. Methods Twenty-four mice were randomly divided into control group (n = 8), tolerance model group (n = 8) and electroacupuncture intervention group (n = 8). Tolerance model group, electroacupuncture intervention group, subcutaneous injection of morphine hydrochloride injection to establish morphine tolerance model. The intervention group was treated with bilateral "Shenshu" point (BL 23) every morning after morphine injection. The latent period of photothermal tail flick was measured on the 7th day of the 1st day. After the pain threshold was measured, the tissues of superior optic chiasma (Suprachiasmatic nucleus,SCN) were taken and the expression of Per1,Per2 gene in SCN was detected by fluorescence quantitative PCR (RT-PCR). Results (1) pain threshold: the pain threshold of tolerance model group and electroacupuncture intervention group was significantly higher than that of initial pain threshold after 5 days of morphine injection. The pain threshold of electroacupuncture intervention group was different from that of initial pain threshold on the 7th day. (2) spontaneous circadian activity rhythm: compared with the blank control group, the mice cycle and total activity period in the model group were higher than those in the control group. There were differences in amplitude results between blank control group, tolerance model group and electroacupuncture intervention group before and after the experiment. (3) the expression level of Per1,Per2 gene in SCN: compared with the blank control group, the relative expression of Per1,Per2 gene in the tolerance model group was down-regulated, and the results were significantly different between the electroacupuncture intervention group and the tolerance model group. Conclusion the electroacupuncture intervention group can decrease the pain threshold of morphine tolerance mice by up-regulating the expression of Rhythm related gene Per1,Per2 in morphine tolerant mice. Delay the formation of tolerance and correct the biological rhythmic disorder in which the amplitude is reduced to performance.
【作者单位】： 成都中医药大学;
【基金】：国家自然基金面上项目(No.81373739) 四川省教育厅课题(No.12ZA035;No.15ZB0089) 成都中医药大学科技发展基金面上项目(No.ZRMS201359;No.ZRQN1613)
【分类号】：R245
，
本文编号：2226315
[Abstract]:Objective to observe the effects of electroacupuncture intervention on the biological rhythm and related clock gene Per1 and Per2 expression in morphine-induced mice. Methods Twenty-four mice were randomly divided into control group (n = 8), tolerance model group (n = 8) and electroacupuncture intervention group (n = 8). Tolerance model group, electroacupuncture intervention group, subcutaneous injection of morphine hydrochloride injection to establish morphine tolerance model. The intervention group was treated with bilateral "Shenshu" point (BL 23) every morning after morphine injection. The latent period of photothermal tail flick was measured on the 7th day of the 1st day. After the pain threshold was measured, the tissues of superior optic chiasma (Suprachiasmatic nucleus,SCN) were taken and the expression of Per1,Per2 gene in SCN was detected by fluorescence quantitative PCR (RT-PCR). Results (1) pain threshold: the pain threshold of tolerance model group and electroacupuncture intervention group was significantly higher than that of initial pain threshold after 5 days of morphine injection. The pain threshold of electroacupuncture intervention group was different from that of initial pain threshold on the 7th day. (2) spontaneous circadian activity rhythm: compared with the blank control group, the mice cycle and total activity period in the model group were higher than those in the control group. There were differences in amplitude results between blank control group, tolerance model group and electroacupuncture intervention group before and after the experiment. (3) the expression level of Per1,Per2 gene in SCN: compared with the blank control group, the relative expression of Per1,Per2 gene in the tolerance model group was down-regulated, and the results were significantly different between the electroacupuncture intervention group and the tolerance model group. Conclusion the electroacupuncture intervention group can decrease the pain threshold of morphine tolerance mice by up-regulating the expression of Rhythm related gene Per1,Per2 in morphine tolerant mice. Delay the formation of tolerance and correct the biological rhythmic disorder in which the amplitude is reduced to performance.
【作者单位】： 成都中医药大学;
【基金】：国家自然基金面上项目(No.81373739) 四川省教育厅课题(No.12ZA035;No.15ZB0089) 成都中医药大学科技发展基金面上项目(No.ZRMS201359;No.ZRQN1613)
【分类号】：R245
，
本文编号：2226315
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