牦牛KLF2基因功能区克隆及组织表达分析
发布时间：2018-09-06 16:44
【摘要】：旨在克隆牦牛KLF2(Krupple-like transcription factor 2,Kruppel样转录因子2)基因功能区序列,阐明其组织表达规律,并揭示基因与肌内脂肪含量的相关性。以麦洼牦牛为试验动物,利用RT-PCR方法克隆KLF2基因功能区序列,荧光定量PCR技术检测该基因在各种组织中的表达模式。结果表明,获得牦牛KLF2基因序列454bp(GenBank登陆号:KX395178),包括KLFs家族典型的3个锌指结构域;KLF2mRNA在牦牛的肺脏和脂肪组织中存在高水平表达,极显著高于其他组织(P0.01);KLF2 mRNA的表达水平与背最长肌IMF(Intramuscular Fat,肌内脂肪)含量呈正相关(P0.01,R=0.885)。结果推测KLF2可能作为牦牛肉质性状的候选基因。
[Abstract]:The aim of this study was to clone the functional region of yak KLF2 (Krupple-like transcription factor 2 Kruppel like transcription factor 2) gene, to elucidate its tissue expression and to reveal the correlation between gene and intramuscular fat content. The functional region of KLF2 gene was cloned from Maiwa yak by RT-PCR method and the expression pattern of the gene in various tissues was detected by fluorescence quantitative PCR. The results showed that 454bp (GenBank accession number: KX395178) of yak KLF2 gene, including three zinc-finger domains of KLFs family, was highly expressed in the lung and adipose tissue of yaks. The expression level of KLF2 mRNA was significantly higher than that of other tissues (P0.01). There was a positive correlation between the expression of KLF2 mRNA and the content of intramuscular fat (IMF (Intramuscular Fat,) in the longissimus dorsi muscle (P0.01). Results it was speculated that KLF2 might be a candidate gene for quality traits of yak.
【作者单位】： 西南民族大学生命科学与技术学院;
【基金】：[基金项目]四川省青年科技创新团队(2015TD0025)
【分类号】：Q78;S823.85
，
本文编号：2226944
[Abstract]:The aim of this study was to clone the functional region of yak KLF2 (Krupple-like transcription factor 2 Kruppel like transcription factor 2) gene, to elucidate its tissue expression and to reveal the correlation between gene and intramuscular fat content. The functional region of KLF2 gene was cloned from Maiwa yak by RT-PCR method and the expression pattern of the gene in various tissues was detected by fluorescence quantitative PCR. The results showed that 454bp (GenBank accession number: KX395178) of yak KLF2 gene, including three zinc-finger domains of KLFs family, was highly expressed in the lung and adipose tissue of yaks. The expression level of KLF2 mRNA was significantly higher than that of other tissues (P0.01). There was a positive correlation between the expression of KLF2 mRNA and the content of intramuscular fat (IMF (Intramuscular Fat,) in the longissimus dorsi muscle (P0.01). Results it was speculated that KLF2 might be a candidate gene for quality traits of yak.
【作者单位】： 西南民族大学生命科学与技术学院;
【基金】：[基金项目]四川省青年科技创新团队(2015TD0025)
【分类号】：Q78;S823.85
，
本文编号：2226944
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