过量表达水稻EPFL家族基因影响拟南芥气孔的发育

发布时间：2018-09-06 20:13

【摘要】：气孔由两个保卫细胞围合而成是植物重要的与外界气孔交换的器官,其在发育过程中受到环境与内部因素的控制,表皮原母细胞由一次不对称分裂开始经过一系列分化,最终形成2个保卫细胞并形成气孔。最近在对EPFL家族基因(epidermis patenting factor like,EPFLs)研究中,发现EPFLs编码的小肽可能在气孔发育中起到调节作用,特别是对气孔密度及在气孔系细胞发生与发展都有调节作用。本研究利用生物信息学方法,比对了水稻(Oryza sativa)基因组,找到与拟南芥(Arabidopsis thaliana)同源的水稻EPFLs基因,构建载体转化拟南芥,以拟南芥为表型研究对象,研究水稻EPFLs在调控气孔形成过程中可能起到的作用,研究发现水稻EPFLs也具有调节气孔发育的作用,其中与Stomagen同源的Os01g0914400水稻基因的表型与拟南芥Stomagen表型非常相似,过表达都增加了气孔的数量与密度,水稻EPFLs中有3个基因Os04g0637-300、Os03g0672500、Os04g0457700转化拟南芥后表现气孔密度显著减少,研究说明水稻中也有调节气孔发育的相关基因,可能对水稻气孔发育与分布密度有影响作用。
[Abstract]:Stomata are surrounded by two guard cells, which are an important organ of stomatal exchange between plants and the outside world. They are controlled by environment and internal factors during their development. Epidermal protoblasts begin to undergo a series of differentiation by a single asymmetric division. Finally, two guard cells and stomata were formed. In recent studies of EPFL family gene (epidermis patenting factor like,EPFLs, it has been found that the small peptides encoded by EPFLs may play a regulatory role in stomatal development, especially in stomatal density and the genesis and development of stomatal cells. In this study, the (Oryza sativa) genome of rice was compared by bioinformatics, and the rice EPFLs gene homologous to Arabidopsis (Arabidopsis thaliana) was found, and the vector was constructed to transform Arabidopsis thaliana. The phenotype of Arabidopsis was studied. To study the role of rice EPFLs in regulating stomatal formation, it was found that rice EPFLs could also regulate stomatal development, and the phenotype of Os01g0914400 gene, which is homologous to Stomagen, was very similar to that of Arabidopsis thaliana Stomagen. Overexpression increased the number and density of stomata, and three Os04g0637-300,Os03g0672500,Os04g0457700 genes in rice EPFLs transformed Arabidopsis thaliana showed a significant decrease in stomatal density. It may have an effect on stomatal development and distribution density of rice.
【作者单位】：新疆农业职业技术学院;中科院上海生命科学院植物生理生态研究所;
【基金】：新疆农业职业技术学院基金项目(XJNZKJ2014001)资助
【分类号】：Q943.2

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