延边黄牛黑皮质素受体-4基因多态性及其与肉质性状的相关性分析

发布时间：2018-09-07 16:18

【摘要】：试验旨在探索调控肉牛脂肪沉积的关键基因对肉质性状的影响,筛选影响肉牛育肥性状的候选基因。本研究以157头24月龄、体重相近的延边黄牛为研究对象,利用PCR扩增直接测序的方法,分析了延边黄牛黑皮质素受体-4(melanocortin-4receptor,MC4R)基因的SNPs及其与肉质性状的相关性。结果发现,延边黄牛MC4R基因在1 069bp处存在C→G突变,导致L(亮氨酸)转变为V(缬氨酸),引起蛋白质二级结构在166、243、280位氨基酸处出现α螺旋、β折叠丢失,导致三级结构中β转角的空间位置改变。经关联分析表明,该位点与延边黄牛背膘厚、亮度、黄度都存在不同程度的相关,其中GG基因型背膘厚与屠宰后黄度(3d)显著高于CC和CG基因型(P0.05);GG、CG基因型屠宰后亮度显著高于CC基因型(P0.05)。说明该位点与延边黄牛肉质性状存在显著相关,该突变位点可作为潜在的分子标记。
[Abstract]:The aim of the experiment was to explore the effect of key genes regulating fat deposition in beef cattle on meat quality traits and to screen candidate genes that affect fattening traits of beef cattle. In this study, 157 24 month-old Yanbian cattle with similar body weight were used to analyze the relationship between the SNPs of melanin receptor 4 (melanocortin-4receptor,MC4R) gene and meat quality of Yanbian cattle by PCR amplification and direct sequencing. The results showed that there was a C-G mutation in the MC4R gene of Yanbian cattle at 1 069bp, which led to the transformation of L (leucine) to V (valine), the formation of 伪 helix and the loss of 尾 folding in the protein secondary structure at 166243280 amino acids. This results in the change of the spatial position of the 尾 rotation angle in the tertiary structure. The correlation analysis showed that this locus was related to the backfat thickness, brightness and yellowness of Yanbian yellow cattle to some extent. The backfat thickness and post-slaughter yellowness of GG genotype were significantly higher than those of CC and CG genotypes (P0.05). The results showed that the mutant locus could be used as a potential molecular marker for the meat quality traits of Yanbian cattle.
【作者单位】：延边大学农学院;
【基金】：吉林省科技发展计划项目(20140201004NY)
【分类号】：S823.81

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