倭竹族14-3-3非ε类基因编码区单核苷酸多态性

发布时间：2018-09-10 06:20

【摘要】：为研究倭竹族Shibataeeae中14-3-3非ε类基因的结构及其编码区单核苷酸多态性,分别从倭竹族5个不同属的代表种中获得14-3-3b,14-3-3c,14-3-3e,14-3-3f的基因全长。这些基因均包含5个外显子和4个内含子,编码序列(CDS)大小为771~789 bp。14-3-3b,14-3-3c,14-3-3e和14-3-3f编码区序列中分别有21,19,26和17个单核苷酸多态性(SNP)位点,SNP频率分别为1SNP/38 bp,1SNP/41 bp,1SNP/30 bp,1SNP/46 bp,基因核苷酸多样性(π)值分别为0.012 17,0.011 83,0.015 21,0.010 38。同一基因在不同竹种间氨基酸序列一致性均达99%以上,毛竹Phyllostachys edulis Pe14-3-3b,Pe14-3-3c,Pe14-3-3e,Pe14-3-3f蛋白的理化性质非常相似,均为亲水性蛋白,含量较多的是丙氨酸(ala),谷氨酸(glu),亮氨酸(leu),等电点为4.75~4.90,这4个蛋白的二级结构也非常相似,α-螺旋结构含量最高,无规则卷曲其次,β-转角和延伸链很少。上述研究为进一步探索竹类植物14-3-3生物学功能奠定基础。
[Abstract]:In order to study the structure and coding region single nucleotide polymorphism of 14-3-3 non-蔚 gene in Shibataeeae of Bamboo, the full length of 14-3-3bSE-14-3-3f gene was obtained from 5 representative species of different genera of Bamboo family. These genes contain five exons and four introns. The nucleotide diversity (蟺) of the coding sequence (CDS) was 771 ~ 789 bp.14-3-3b,14-3-3c,14-3-3e and 14-3-3f, respectively. The frequency of SNPs in the coding region was 21 / 19 / 26 and 17 / 17, respectively. The nucleotide diversity (蟺) of the 1SNP/38 bp,1SNP/41 bp,1SNP/30 bp,1SNP/46 bp, gene was 0.012 / 711 / 830.015 / 21 and 0.010 / 38, respectively. The amino acid sequence of the same gene was more than 99% in different bamboo species. The physicochemical properties of Phyllostachys edulis Pe14-3-3b,Pe14-3-3c,Pe14-3-3e,Pe14-3-3f protein in Phyllostachys pubescens were very similar and they were hydrophilic proteins. The content of alanine (ala), glutamate (glu), leucine (leu), was 4.75 ~ 4.90. The secondary structure of the four proteins was similar, the content of 伪 -helix structure was the highest, the irregular curl was the second, and the 尾 -rotation angle and extension chain were few. The above studies lay a foundation for further exploring the biological functions of 14-3-3 bamboos.
【作者单位】：浙江农林大学亚热带森林培育国家重点实验室培育基地;
【基金】：国家自然科学基金资助项目(30901155) 浙江省自然科学基金资助项目(Y307499)
【分类号】：S795

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