不同断奶日龄对仔猪肝脏热休克蛋白基因表达的影响

发布时间：2018-09-12 20:25

【摘要】：为研究不同断奶日龄对仔猪肝脏热休克蛋白70(Hsp70)、热休克蛋白90(Hsp90)和热休克蛋白27(Hsp27)基因表达的影响,试验选取体重相近的24头健康杜长大仔猪,按4种断奶日龄(14日龄、21日龄、28日龄和35日龄)随机分为4个处理组,每组包含6个重复,仔猪42日龄时全部处死取样,用RT-PCR方法检测肝脏中上述基因的相对表达水平。结果表明,在4组断奶日龄仔猪肝脏中,14日龄断奶组Hsp70mRNA的相对表达量显著高于28和35日龄断奶组(P0.05),显著低于21日龄断奶组(P0.05);14和21日龄断奶组Hsp90mRNA的相对表达量均显著高于28和35日龄断奶组(P0.05);14日龄断奶组的Hsp27mRNA的相对表达量均显著高于21、28和35日龄断奶组(P0.05)。结果提示,断奶日龄显著影响仔猪热休克蛋白基因的表达,且随断奶日龄的推迟,HSPs mRNA的相对表达量呈下降趋势。
[Abstract]:In order to study the effects of different weaning ages on the expression of heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90) and heat shock protein 27 (Hsp27) in liver of piglets, 24 healthy adult piglets of similar body weight were selected to study the expression of heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90) and heat shock protein 27 (Hsp27). According to four weaning days (14 days old, 21 days old, 28 days old and 35 days old), they were randomly divided into four treatment groups. Each group contained 6 replicates. All piglets were killed and sampled at 42 days of age. The relative expression level of the above genes in liver was detected by RT-PCR method. The results show that The relative expression of Hsp70mRNA in liver of 14 day weaning group was significantly higher than that of 28 and 35 day weaning group (P0.05), and significantly lower than that of 21 day old weaning group (P0.05). The relative expression of Hsp90mRNA in 14 and 21 day weaning group was significantly higher than that in 28 and 35 day weaning group. The relative expression of Hsp27mRNA in 35-day-old weaning group (P0.05) was significantly higher than that in 21-day-28 and 35-day-old weaning group (P0.05). The results suggested that the expression of heat shock protein (HSPs) genes in piglets was significantly affected by weaning age, and the relative expression of HSPs mRNA decreased with the delay of weaning day.
【作者单位】：天津农学院动物科学与动物医学学院;南京农业大学畜牧学博后科研流动站;
【基金】：天津市自然科学基金项目(13JCYBJC25300) 天津市高校中青年骨干创新人才培养计划资助项目(津教委人[2013]13号) 天津市“131”创新型人才培养工程第一层次人选资助项目(津人才[2016]2号)
【分类号】：S828

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