拟南芥莲座叶片发育过程中P1基因的表达特性

发布时间：2018-09-13 15:04

【摘要】：该实验对CDF1类似蛋白基因(P1)在拟南芥叶片发育不同阶段的定量PCR结果显示,P1基因在拟南芥叶片发育的所有时期均可表达,但在茎生叶和衰老叶中的表达水平明显高于成熟叶和幼叶。GUS报告基因表达的组织化学染色结果显示,P1启动子在拟南芥叶片中有较高的驱动活性;在营养生长阶段的幼苗和植株(4~5周)的所有叶片中均能检测到GUS表达,但在植株转入生殖生长阶段后(6周及以后),GUS表达主要集中在逐渐衰老的叶中,并随着叶片衰老程度加剧GUS染色程度也越深,这一结果与GUS荧光定量检测结果一致。通过分析P1基因启动子上可能存在的顺式调控元件,发现茉莉酸甲酯、热压、干旱和水杨酸等均能够引起叶片衰老调控元件的响应,证实P1的表达受到这些因素的调控。研究表明,P1在拟南芥莲座叶片中很可能参与了对上游衰老信号的响应,该研究结果为进一步探究P1在叶片衰老过程中的分子功能验证奠定了基础。
[Abstract]:The quantitative PCR results of CDF1 like protein gene (P1) at different stages of leaf development in Arabidopsis thaliana showed that it could be expressed at all stages of leaf development in Arabidopsis thaliana. But the expression level in stem leaves and senescent leaves was significantly higher than that in mature leaves and young leaves. The histochemical staining results showed that the promoter P1 had higher driving activity in Arabidopsis thaliana leaves. The expression of GUS was detected in all leaves of seedlings and plants (4 ~ 5 weeks) in vegetative growth stage, but the expression of Gus was mainly in the gradually aging leaves (6 weeks and later) after the plant was transferred to reproductive growth stage. With the increase of leaf senescence, the degree of GUS staining was deeper, which was consistent with the results of GUS fluorescence quantitative detection. By analyzing the possible cis-regulatory elements in P1 gene promoter, it was found that methyl jasmonate, hot pressure, drought and salicylic acid could all cause the response of leaf senescence regulatory elements, which confirmed that the expression of P1 was regulated by these factors. It is suggested that P1-1 may be involved in the response to upstream senescence signals in Arabidopsis thaliana leaves. The results provide a basis for further exploring the molecular function of P1 in leaf senescence.
【作者单位】：福建农林大学生命科学院分子细胞与系统生物学中心;福建国际旅行卫生保健中心;
【基金】：国家自然科学基金(31400260,31470383) 福建省自然科学基金(2015J01082)
【分类号】：Q943.2

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