棉花适宜机采相关性状的SSR标记关联分析及优异等位基因挖掘

发布时间：2018-09-14 13:19

【摘要】：棉花机械采收对品种的生育期、株型及对脱叶剂敏感度有较高的要求。本研究利用覆盖全基因组有多态性的214对SSR标记对118份含有一个或多个机采性状的种质资源的株高、始节高、始节位、第一果枝平均长度、生育期及脱叶率6个机采相关性状进行关联分析。利用Structure 2.3.1软件进行群体结构分析,并结合2年2点12个重复的田间表型数据,采用Tassel 5.0软件的混合线性模型MLM关联定位。结果检测到460个等位基因,涉及905个基因型,基因多样性指数平均为0.5151,PIC值平均为0.4587,基因多样性指数和PIC值都大于平均数的标记有99个,占总标记数的46.3%,说明该批SSR标记具有较多的等位变异数和较高的遗传多样性。群体结构分析将118份供试材料划分为4个亚群,结果显示各类群中材料与地理来源无对应关系。关联分析结果显示4种环境中,在显著条件下(P0.05),共检测到124个与6个机采相关性状相关的位点,对表型变异解释率范围为2.23%~14.15%;其中在极显著条件下(P0.01),共检测到20个与机采相关性状相关的位点,对表型变异解释率范围为4.84%~14.15%。基于本研究的结果,鉴定出典型的载体材料11份,分别为系7、金垦9号、Y11、豫棉18、AY-4、K2、朝阳棉2号、DZ22、中棉所43、C2和关农长早B14。以上发掘出的控制棉花适宜机采性状的优异等位基因及优异亲本资源,可为机采棉的分子辅助选择育种提供理论依据。
[Abstract]:Mechanical harvesting of cotton has high requirements for growth period, plant type and sensitivity to defoliant. In this study, 214 pairs of SSR markers covering the whole genome were used to study the plant height, initial node position and average length of the first fruit branch of 118 germplasm resources with one or more mechanically collected traits. Correlation analysis was carried out for 6 related characters of growth period and defoliation rate. The population structure was analyzed by Structure 2.3.1 software, and combined with 2 points and 12 repeated field phenotypic data for 2 years. The mixed linear model (MLM) of Tassel 5.0 was used to locate the population. Results 460 alleles were detected, involving 905 genotypes. The average genetic diversity index was 0.5151. The average PIC value was 0.4587. There were 99 markers whose genetic diversity index and PIC value were higher than the average. 46.3% of the total number of markers showed that the SSR markers had more allelic variances and higher genetic diversity. 118 tested materials were divided into 4 subgroups by population structure analysis. The results showed that there was no corresponding relationship between the materials and geographical sources. The results of correlation analysis showed that 124 loci related to 6 machine-picking related traits were detected under significant conditions (P0.05) in four environments. The interpretation rate of phenotypic variation was 2.23 and 14.15, among which 20 loci were detected under extremely significant conditions (P0.01), and the interpretation rate of phenotypic variation was 4.84% 14.15%. Based on the results of this study, 11 typical carrier materials were identified, which were line 7, Jinken 9, Y11, Yumian18AY-4K2, Chaoyang Mian2 DZ22, Zhongmiansuo 43C _ 2 and Guan Nongzao B14, respectively. The excellent alleles and parent resources to control cotton suitable for mechanical harvesting can provide theoretical basis for molecular assisted selection breeding of mechanically harvested cotton.
【作者单位】：新疆农垦科学院棉花研究所/农业部西北内陆区棉花生物学与遗传育种重点实验室;新疆农业大学农学院;
【基金】：新疆生产建设兵团博士资金项目(2013BB001);新疆生产建设兵团重大科技项目(2016AA001-1) 国家自然科学基金项目(31260340)资助~~
【分类号】：S562

【相似文献】