水稻黄叶突变体yl的遗传分析与基因定位
发布时间：2018-09-18 06:58
【摘要】：[目的]水稻叶片作为重要的光合器官,是作物产量的形成基础。叶色突变体不仅可以作为育种标记,还是研究叶绿体发育和培育高光效水稻品种的先决条件之一。[方法]从籼稻品种‘9311’的~(60)Co-γ射线辐射诱变突变体库中筛选获得了1个水稻黄叶突变体yl。利用yl/‘宁粳4号’杂交的F2群体进行基因定位。[结果]与野生型相比,yl突变体的幼叶表现出明显的黄化,叶绿素a、叶绿素b、类胡萝卜素的含量下降。突变体叶绿体中类囊体片层结构排列松散。与野生型相比,yl突变体的每穗粒数、结实率和千粒质量下降。该性状受1对隐性核基因控制。该基因初步定位于第9染色体长臂上,进一步开发分子标记把定位区间缩小在14.5 kb内,该区域包含3个候选基因。经测序比对分析发现,yl突变体的第3个ORF的第5外显子发生碱基突变(2131CTA→G),从而造成蛋白第233位氨基酸发生改变并导致翻译提前终止,形成了1个包含原有232个氨基酸和16个新形成氨基酸残基的蛋白,该基因与已报道的水稻黄叶基因YLC1是等位基因。[结论]本研究明确了YLC1参与叶绿素的合成,为叶绿体色素合成和叶绿体发育的研究提供了基础。
[Abstract]:Objective: as an important photosynthetic organ, rice leaf is the basis of crop yield. Leaf color mutants can be used not only as breeding markers, but also as a prerequisite for chloroplast development and breeding of high light efficiency rice varieties. [methods] A rice yellow leaf mutant yl. was obtained from the mutant library of ~ (60) Co- 纬 -ray radiation induced by indica rice cultivar Zeng9311'. The F 2 population of yl/' Ningjing 4 'crossing was used to localize the gene. [results] compared with the wild type, the young leaves of the mutant showed obvious yellowing, and the contents of chlorophyll a, chlorophyll b and carotenoid decreased. Thylakoid lamellae in mutant chloroplasts is loosely arranged. Compared with wild type, the number of kernels per panicle, the seed setting rate and the weight of 1000 kernels decreased. This trait is controlled by a pair of recessive nuclear genes. The gene was preliminarily located on the long arm of chromosome 9 and further developed molecular markers to narrow the localization range to 14. 5 kb. The region contains three candidate genes. Sequencing analysis revealed that the 2131CTA G), of the third ORF of the mutants had a base mutation (2131CTA G), which resulted in the change of amino acid at the 233rd position of the protein and the early termination of translation. A protein containing 232 amino acids and 16 newly formed amino acid residues was formed. The gene is allelic to the reported rice yellow leaf gene YLC1. [conclusion] YLC1 is involved in the synthesis of chlorophyll, which provides a basis for the study of chloroplast pigment synthesis and chloroplast development.
【作者单位】： 南京农业大学作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心;
【基金】：国家科技支撑计划项目(2015BAD01B02-7)
【分类号】：S511;Q943.2
，
本文编号：2247137
[Abstract]:Objective: as an important photosynthetic organ, rice leaf is the basis of crop yield. Leaf color mutants can be used not only as breeding markers, but also as a prerequisite for chloroplast development and breeding of high light efficiency rice varieties. [methods] A rice yellow leaf mutant yl. was obtained from the mutant library of ~ (60) Co- 纬 -ray radiation induced by indica rice cultivar Zeng9311'. The F 2 population of yl/' Ningjing 4 'crossing was used to localize the gene. [results] compared with the wild type, the young leaves of the mutant showed obvious yellowing, and the contents of chlorophyll a, chlorophyll b and carotenoid decreased. Thylakoid lamellae in mutant chloroplasts is loosely arranged. Compared with wild type, the number of kernels per panicle, the seed setting rate and the weight of 1000 kernels decreased. This trait is controlled by a pair of recessive nuclear genes. The gene was preliminarily located on the long arm of chromosome 9 and further developed molecular markers to narrow the localization range to 14. 5 kb. The region contains three candidate genes. Sequencing analysis revealed that the 2131CTA G), of the third ORF of the mutants had a base mutation (2131CTA G), which resulted in the change of amino acid at the 233rd position of the protein and the early termination of translation. A protein containing 232 amino acids and 16 newly formed amino acid residues was formed. The gene is allelic to the reported rice yellow leaf gene YLC1. [conclusion] YLC1 is involved in the synthesis of chlorophyll, which provides a basis for the study of chloroplast pigment synthesis and chloroplast development.
【作者单位】： 南京农业大学作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心;
【基金】：国家科技支撑计划项目(2015BAD01B02-7)
【分类号】：S511;Q943.2
，
本文编号：2247137
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