A-FABP基因多态性与猪肌内脂肪含量的相关性分析

发布时间：2018-09-18 09:30

【摘要】：为了研究猪肌内脂肪(IMF)含量与相关基因多态性的关系,试验采用PCR-SSCP技术检测了75头大河乌猪和41头长白猪脂肪细胞型脂肪酸结合蛋白(A-FABP)基因的第1内含子区的遗传变异,并用最小二乘法模型分析了A-FABP基因对猪肌内脂肪含量的遗传效应。结果表明:在大河乌猪和长白猪A-FABP基因第1内含子中均存在多态性,共发现5个等位基因(A、B、C、D、E),6种基因型(AB基因型、BB基因型、BC基因型、BD基因型、CC基因型、CE基因型)。大河乌猪肌内脂肪含量BD基因型显著高于其他5种基因型(P0.05),BC基因型显著低于其他5种基因型(P0.05),BD基因型与其他5种基因型之间最小二乘均值差异显著(P0.05),BD基因型对PCR-SSCP的最小二乘均值最大,证明BD基因型在该位点对肌内脂肪含量的效应值最大。长白猪肌内脂肪含量CC基因型高于其他5种基因型,BC基因型显著低于其他5种基因型(P0.05),最小二乘均值6种基因型之间差异均不显著(P0.05),CC基因型对PCR-SSCP的最小二乘均值最大,证明CC基因型在该位点对肌内脂肪含量的效应值最大。因此,可以通过提高BD基因型的频率来增加大河乌猪肌内脂肪含量,达到改善大河乌猪猪肉品质的目的。
[Abstract]:In order to study the relationship between intramuscular fat (IMF) content and related gene polymorphism, the genetic variation of the first intron of adipocyte fatty acid binding protein (A-FABP) gene in 75 Dahewu pigs and 41 Landrace pigs was detected by PCR-SSCP technique. The genetic effect of A-FABP gene on intramuscular fat content in pigs was analyzed by least square method. The results showed that there were polymorphisms in intron 1 of A-FABP gene in Dahewu pig and Landrace pig, and six genotypes (AB genotype, BB, B, C, C and C) were found. The intramuscular fat content of Dahewu pig was significantly higher than that of the other 5 genotypes (P0.05). (P0.05) the least square mean value (LMS) of the BDD genotype was significantly lower than that of the other 5 genotypes (P0.05). (P0.05) the difference of the least square mean value between the BD genotype and the other 5 genotypes was significant (P0.05). Has the largest mean of least squares based on The results showed that BD genotype had the largest effect on intramuscular fat content at this site. The intramuscular fat content of Landrace pigs was significantly lower than that of the other 5 genotypes (P0.05), and the least square mean (LSE) was not significant among the six genotypes (P0.05). The results showed that CC genotype had the largest effect on intramuscular fat content at this site. Therefore, the intramuscular fat content of Dahewu pig can be increased by increasing the frequency of BD genotype, and the pork quality of Dahewu pig can be improved.
【作者单位】：云南农业大学云南省动物营养与饲料重点实验室;
【基金】：国家自然科学基金项目(31260592;31060331)
【分类号】：S828

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