自噬相关基因Beclin1和LC3在卵巢浆液性癌中的表达及临床意义

发布时间：2018-09-18 13:13

【摘要】：目的检测Beclin1和LC3在卵巢浆液性癌(ovarian serous carcinoma,OSC)中的表达,探讨两者与OSC临床病理特征及预后的关系。方法采用免疫组化Max Vision两步法检测63例OSC和20卵巢浆液性囊腺瘤组织中Beclin1和LC3蛋白的表达,分析两者与OSC临床病理特征及预后的关系。应用RT-PCR和Western blot法检测10例新鲜OSC组织和相应癌旁组织中Beclin1和LC3 mRNA和蛋白的表达。结果在OSC组织中Beclin1和LC3蛋白阳性率(36.51%、33.33%)均低于卵巢浆液性囊腺瘤组织(65.00%、60.00%),差异有统计学意义(P=0.025,P=0.034)。Beclin1蛋白表达与临床FIGO分期和病理分级有关(P=0.001,P=0.001),与患者年龄、发病部位、肿瘤大小及有无盆腔淋巴结转移无关(P0.05)。LC3蛋白表达与临床FIGO分期和盆腔淋巴结转移有关(P=0.013,P=0.041),与患者年龄、发病部位、肿瘤大小和病理分级均无关(P0.05)。Beclin1和LC3在OSC中的表达呈显著正相关(r_s=0.373,P=0.03)。10例新鲜OSC组织中Beclin1和LC3 mRNA的相对表达量(0.581±0.091、0.650±0.090)低于癌旁组织,两者比较差异有统计学意义(t=8.083,t=6.614;P=0.016,P=0.022)。新鲜OSC组织中Beclin1和LC3蛋白的相对表达量明显低于癌旁组织,两者比较差异有统计学意义(P0.05)。Kaplan-Meier生存分析表明,Beclin1和LC3表达与OSC患者预后相关(P=0.028 3,P=0.018 5)。结论自噬相关基因Beclin1和LC3在OSC组织中的表达下降,导致自噬功能减弱,可能与OSC的发生、发展和预后不良相关。
[Abstract]:Objective to detect the expression of Beclin1 and LC3 in ovarian serous carcinoma (ovarian serous carcinoma,OSC) and to explore the relationship between them and the clinicopathological features and prognosis of OSC. Methods the expression of Beclin1 and LC3 in 63 cases of OSC and 20 ovarian serous cystadenomas were detected by immunohistochemical Max Vision two-step method. The relationship between the expression of Beclin1 and LC3 and the clinicopathological features and prognosis of OSC was analyzed. RT-PCR and Western blot were used to detect the expression of Beclin1, LC3 mRNA and protein in 10 fresh OSC tissues and corresponding adjacent tissues. Results the positive rate of Beclin1 and LC3 protein in OSC tissues (36.51%) was lower than that in ovarian serous cystadenoma tissues (65.00%). The difference was statistically significant (P0. 025%, P0. 034). Beclin1 protein expression was correlated with clinical FIGO stage and pathological grade (P0. 001). There was no correlation between tumor size and pelvic lymph node metastasis (P0.05). LC3 protein expression was correlated with clinical FIGO stage and pelvic lymph node metastasis (P0. 013). There was no significant correlation between tumor size and pathological grade (P0.05). The expression of Beclin1 and LC3 in OSC was positively correlated (RSP: 0.373 P0. 03). The relative expression of Beclin1 and LC3 mRNA in 10 fresh OSC tissues (0.581 卤0.091 卤0.090) was significantly lower than that in para-cancerous tissues (t8.083t6.614P ~ (0.016) P0. 022). The relative expression of Beclin1 and LC3 protein in fresh OSC tissues was significantly lower than that in paracancerous tissues (P0.05). Kaplan-Meier survival analysis showed that the expression of Beclin1 and LC3 was correlated with the prognosis of OSC patients (P0.028 3, P0. 018 5). Conclusion the decreased expression of autophagy related genes Beclin1 and LC3 in OSC may lead to the decrease of autophagy function, which may be related to the occurrence, development and poor prognosis of OSC.
【作者单位】：福建医科大学省立临床医学院/福建省立医院病理科;福建医科大学省立临床医学院/福建省立医院药剂科;福建医科大学省立临床医学院/福建省立医院妇科;
【基金】：福建省自然科学基金(2015J01424) 福建省立医院院内优秀青年基金(2014074)
【分类号】：R737.31

【相似文献】