蒙古沙冬青J蛋白基因AmJ20的克隆与表达分析
发布时间：2018-09-18 20:17
【摘要】：DNA J蛋白是植物中广泛存在的一类分子伴侣,对于维持正常和逆境条件下蛋白质的稳态具有重要作用。我们在前期通过对强抗逆植物蒙古沙冬青进行转录组分析,发现一个寒旱诱导表达的J蛋白即Am J20编码序列,但其3'端编码区不完整。本研究通过3'RACE获得其c DNA全长序列(800 bp),推测其编码蛋白由179个氨基酸残基组成,含一个典型的J结构域,属于Ⅲ型J蛋白,此外还具有叶绿体和细胞核双定位信号;利用半定量RT-PCR方法检测该基因在野外生长植株中的时空表达模式,发现其在春、夏、秋季只有微量表达,甚至检测不到转录本,而在严冬时节高表达,此外在叶片中的表达量明显高于根、嫩枝、花蕾和幼嫩果荚;利用RT-PCR方法克隆到该基因编码区c DNA并将其插入到植物超表达载体上,为通过转基因植物分析该基因的功能提供了理论依据。
[Abstract]:DNA J protein is a widespread molecular chaperone in plants, which plays an important role in maintaining the homeostasis of proteins under normal and stress conditions. By transcriptome analysis of the strong resistant plant, Aconitum mongolica, we found that a cold-drought induced J protein called Am J20 coding sequence was found in the early stage, but its 3 'terminal coding region was incomplete. In this study, the full-length sequence of c DNA was obtained by 3'RACE (800 bp), conjectured that the encoding protein consisted of 179 amino acid residues, containing a typical J domain, belonging to type 鈪，
本文编号：2249004
[Abstract]:DNA J protein is a widespread molecular chaperone in plants, which plays an important role in maintaining the homeostasis of proteins under normal and stress conditions. By transcriptome analysis of the strong resistant plant, Aconitum mongolica, we found that a cold-drought induced J protein called Am J20 coding sequence was found in the early stage, but its 3 'terminal coding region was incomplete. In this study, the full-length sequence of c DNA was obtained by 3'RACE (800 bp), conjectured that the encoding protein consisted of 179 amino acid residues, containing a typical J domain, belonging to type 鈪，
本文编号：2249004
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