裂叶桦和欧洲白桦叶片形态特征及相关基因表达特性比较

发布时间：2018-10-11 10:59

【摘要】：为了探寻欧洲白桦变种裂叶桦的叶缘分裂机制。以2种桦树为研究对象,在叶片形态特征和解剖特征观测的基础上,进行了顶芽及叶片叶尖、裂片及凹缺等不同组织部位相关基因表达定量分析、内源激素IAA含量测定以及IAA活体标记的GUS染色分析。结果表明,裂叶桦叶片两侧叶缘自叶基到叶尖分裂出由大到小3~4个裂片,叶背面的叶脉呈明显的管状隆起,叶宽、平均二级脉间距、主脉横切面积等分别较欧洲白桦大了21.12%、12.64%、21.46%。BpmiR164s及靶基因的组织表达特性分析结果证明,BpmiR164-2及靶基因BpCUC2与裂片的形成关系密切,是裂叶性状产生的主要调控基因。生长素输出载体BpPINs基因的组织表达特性分析结果证明,6个基因中只有BpPIN2和BpPIN5在裂叶桦的叶尖、裂片及顶芽等组织器官中呈现上调表达。内源激素IAA的活体标记发现,在顶芽、幼叶中IAA的含量较高,而在叶片中,叶尖、叶齿及裂片处是激素的高表达部位,利用液相色谱—质谱联用仪(LC-MS)对2种桦树的上述不同组织部位的生长素测定也证明了激素的上述分布特点。综合上述,裂叶桦裂片产生是激素在叶脉、叶缘的极性运输及相关基因调控的结果。
[Abstract]:In order to find out the mechanism of leaf margin division of Betula platyphylla var. Bai Hua in Europe. Based on the observation of morphological and anatomical characteristics of two species of birch, quantitative analysis of gene expression in different tissues such as apical bud, leaf tip, lobes and concave was carried out. The content of endogenous hormone IAA and GUS staining of IAA in vivo were determined. The results showed that the leaves of Betula lobata were separated from the base to the tip, and the veins on the back of the leaf were obviously tubular, with the width of the leaf, and the average secondary vein spacing. The cross sectional area of main veins was 21.12 / 12.64 and 21.46. BpmiR164s and target gene were respectively larger than those of Bai Hua in Europe. The results showed that BpmiR164-2 and target gene BpCUC2 were closely related to the formation of lobes and were the main regulatory genes for the formation of lobes. The results of tissue expression analysis of BpPINs gene of auxin export vector showed that only BpPIN2 and BpPIN5 showed upregulated expression in tissues and organs of Betula lobata such as tip, lobes and terminal buds of Betula lobata. In vivo marker of endogenous hormone IAA, it was found that the content of IAA was higher in terminal bud and young leaf, while in leaf, tip, tooth and lobes were the high expression sites of hormone. The determination of auxin in different tissues of two birch species by liquid chromatography-mass spectrometry (LC-MS) also proved the distribution of hormones. To sum up above, the production of Betula fissure lobes is the result of the polar transport of hormones in leaf veins and margins and the regulation of related genes.
【作者单位】： 东北林业大学林木遗传育种国家重点实验室;
【基金】：国家自然科学基金项目(31370660)
【分类号】：S792.15

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