番茄HSP20基因家族的全基因组鉴定、系统进化及表达分析

发布时间：2018-10-11 12:16

【摘要】：热激蛋白是从细菌到高等真核生物中普遍存在的一种在受到环境胁迫的响应后迅速合成的蛋白。因为小分子热激蛋白的分子量普遍在20k Da左右,故称为HSP20。借助番茄基因组数据库,利用生物信息学方法对HSP20基因家族进行鉴定及分析,结果表明,番茄中至少含有43个HSP20基因,不均匀的分布在番茄的12条染色体上。通过对茄科植物番茄、辣椒、马铃薯HSP20进行系统进化分析发现,三者存在同源关系。对HSP20基因启动子序列分析发现多个响应植物糖代谢和逆境胁迫的顺式作用元件,包括SURE、W-box等。基于RNA-Seq数据库中2种测序番茄(Heinz1706和pimpinellifolium)的表达分析发现,大多数家族成员在果实发育时期表达量高,在其他组织中的表达量相对较低。这些研究结果为鉴定番茄HSP20基因的进化及其功能奠定了基础。
[Abstract]:Heat shock protein (HSPs) is a rapidly synthesized protein from bacteria to higher eukaryotes, which is rapidly synthesized in response to environmental stress. Because the molecular weight of small heat shock protein is generally about 20k Da, it is called HSP20.. With the help of tomato genome database and bioinformatics method, the HSP20 gene family was identified and analyzed. The results showed that there were at least 43 HSP20 genes in tomato, which were unevenly distributed on 12 chromosomes of tomato. Phylogenetic analysis of tomato, capsicum and potato HSP20 showed that there was a homologous relationship among them. HSP20 gene promoter sequence analysis revealed several cis-acting elements, including SURE,W-box, that respond to plant glucose metabolism and stress. Based on the analysis of the expression of two kinds of sequenced tomato (Heinz1706 and pimpinellifolium) in RNA-Seq database, it was found that the expression of most family members was high during fruit development, but relatively low in other tissues. These results laid a foundation for identifying the evolution and function of tomato HSP20 gene.
【作者单位】：沈阳农业大学园艺学院/设施园艺省部共建教育部重点实验室/辽宁省设施园艺重点实验室;
【基金】：国家自然科学基金项目(31672138)
【分类号】：S641.2

【相似文献】