不同茶树品种PPO基因单核苷酸多态性分析

发布时间：2018-10-13 16:39

【摘要】：多酚氧化酶是茶叶加工过程中品质形成的关键酶类,在任何一种茶叶加工过程中都起着至关重要的作用。不同的品种在长期的演化过程中形成了丰富的多酚氧化酶单核苷酸多态性。本研究主要对著名红茶产区选育出的宜兴群体品种与引进的部分优良品种多酚氧化酶基因编码区进行传统克隆测序,研究其单核苷酸多态性,分析不同品种间的遗传距离远近,以及利用不同品种之间的独特SNP位点进行品种鉴别。结果表明:32个品种共发现了120个SNP位点,其中81个为非同义突变,39个同义突变,核苷酸多态性指数pi为0.011 06,基因的单倍型多样性(HD)为1.0,说明多酚氧化酶基因具有较高的遗传多样性。香山早与梅占的遗传距离最大,为0.02,这个两个品种亲缘关系最远,遗传距离最小的是鸿雁12号与白叶一号、湘波绿、玉兰、两两之间遗传距离均为0.001,横岩3号和乌牛早之间遗传距离也为0.001,亲缘关系最近。利用不同品种间的多酚氧化酶基因SNP位点,可以成功鉴别区分开32个品种,研究结果将在茶树遗传育种、利用遗传工程手段提高茶叶品质中具有重大意义。
[Abstract]:Polyphenol oxidase (PPO) is the key enzyme in the process of tea processing and plays an important role in any tea processing. Polyphenol oxidase single nucleotide polymorphisms were formed in different varieties during long-term evolution. In this study, the traditional cloning and sequencing of polyphenol oxidase gene coding region of Yixing population selected from famous black tea production area and some imported fine varieties were carried out, the single nucleotide polymorphism was studied, and the genetic distance between different varieties was analyzed. And the unique SNP loci among different varieties were used to identify the varieties. The results showed that there were 120 SNP loci in 32 cultivars, among which 81 were non-synonymous mutations and 39 were synonymous mutations. The nucleotide polymorphism index (pi) was 0.011 and the haplotype diversity (HD) of the gene was 1.0, which indicated that the polyphenol oxidase gene had high genetic diversity. The genetic distance between Xiangshan Zaozao and Meizhan was the largest (0.02). The genetic distance between the two cultivars was the furthest, and the genetic distance was the least between Hongyan 12 and Baiye No. 1, Xiangbo Green, Magnolia. The genetic distance between the two was 0.001, and the genetic distance between Yokyan No. 3 and Wuniuzao was 0.001. The genetic relationship was the most recent. Using the SNP locus of polyphenol oxidase gene among different varieties, 32 varieties can be distinguished successfully. The results will be of great significance in the genetic breeding of tea plants and the improvement of tea quality by means of genetic engineering.
【作者单位】：西南林业大学园林学院;
【基金】：园林植物与观赏园艺重点实验室平台建设项目(31160177)资助
【分类号】：S571.1

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