八个抗稻瘟病基因在华南籼型杂交水稻中的分布

发布时间：2018-10-16 19:29

【摘要】：【目的】已克隆的稻瘟病抗性基因Pi1、Pik-p、Pik-h、Pi2、Pi9、Piz-t、Pita、Pii对不同稻区的稻瘟病菌表现较广谱的抗性,被广泛应用于水稻抗瘟性育种。为了明确上述抗性基因在华南稻区杂交稻组合中的分布及其组合的抗病有效性,【方法】利用上述8个抗病基因的功能标记,对华南328个杂交稻组合进行了抗瘟基因型分子检测。【结果】抗性基因Pita和Pii分布频率最高,在测试组合中检出率分别为84.76%与67.68%;其次是Pi2与Pik-p,分别为22.87%与13.72%;检出频率较低的是Pi1、Piz-t和Pik-h,分别为5.18%、3.35%与2.13%,检测的品种都不携带抗性基因Pi9。在单个杂交稻组合中,检出的抗瘟基因数量最多是4个。抗病性评价结果表明,杂交稻组合中检出的抗病基因数量越多,其表现为抗病品种的频率就越高;含4个抗病基因的杂交稻组合中,抗病品种所占比率达91.67%。含有不同抗瘟基因的组合表现出不同水平的抗瘟性,其中Pi2与Pi1对华南稻区稻瘟病的抗病性贡献最大,其他抗病基因的贡献大小依次是Pik-h、Pik-p、Pita、Pii与Piz-t。【结论】本研究为华南稻区杂交稻抗病品种基因型的合理布局以及抗瘟基因的应用提供了科学依据。
[Abstract]:[objective] the cloned blast resistance gene (Pi1,Pik-p,Pik-h,Pi2,Pi9,Piz-t,Pita,Pii) was widely used in rice blast resistance breeding. In order to determine the distribution of the above resistance genes in South China hybrid rice combinations and their resistance effectiveness, the functional markers of the above eight resistance genes were used. Genotypes of resistance to blast were detected in 328 hybrid rice combinations in South China. [results] the distribution frequency of Pita and Pii was the highest. In the test combination, the detection rate was 84.76% and 67.68%, followed by Pi2 and Pik-p, 22.87% and 13.72, respectively. The detection frequency of Pi1,Piz-t and Pik-h, were 5.18% and 2.13%, respectively. All the tested varieties did not carry the resistant gene Pi9.. In a single hybrid rice combination, the maximum number of blast resistant genes detected was 4. The results of disease resistance evaluation showed that the more resistant genes were detected in hybrid rice combinations, the higher the frequency of resistant varieties was, and the percentage of resistant varieties in hybrid rice combinations containing 4 resistant genes was 91.67%. Combinations containing different blast resistance genes showed different levels of resistance to blast, in which Pi2 and Pi1 contributed most to the resistance to blast in South China. The contribution of other resistant genes was Pik-h,Pik-p,Pita,Pii and Piz-t. [conclusion] this study provided a scientific basis for the rational distribution of disease resistance genotypes and the application of blast genes in South China hybrid rice.
【作者单位】：广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室;广东省农业技术推广总站;
【基金】：国家重点研发计划项目(2016YFD0300700) 广东省科技计划资助项目(2015B020231003;2016B090918116;2016A050502030) 国家水稻产业技术体系资助项目(CARS-01-24)
【分类号】：S435.111.41

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