紫色黄秋葵转录组功能基因测序及分析

发布时间：2018-10-16 22:17

【摘要】：黄秋葵为药食兼用型植物,富含花青素、多糖、黄酮及萜类等活性成分。为探讨黄秋葵次级代谢过程中这些活性物质合成的遗传基础,以紫色黄秋葵嫩茎叶为材料,采用Illumina Hi Seq 2500高通量转录组测序技术获得23 026 500个短读序,经组装获得42 484个Unigene,平均长度877 bp。31 931个和21 926个Unigene分别在Nr和Swiss Prot数据库有同源比对信息;Unigene与COG、KOG数据库进行比对,根据其功能各分为24类和25类;通过Pfam数据库,所注释到的20 031个Unigene分属于7 277类蛋白结构域;GO注释到18 602个Unigene分为细胞组分、分子功能及生物学过程等三大类的51个功能组,其中大量Unigene与细胞部分、细胞、催化活性、结合活性、代谢进程及细胞进程相关;根据KEGG数据库,可将7 619个Unigene定位到119个代谢途径分支,其中有1、3、35、61、13和70个Unigene分属于花色素苷、黄酮、类黄酮、N-多糖、二萜类和萜类骨架生物合成路径,它们可能参与这些活性物质的生物合成。本研究结果为后续深入开展黄秋葵花青素、黄酮类等物质代谢合成途径及相关功能基因研究奠定了基础。
[Abstract]:Sunflower is a kind of medicinal and food plant, which is rich in anthocyanins, polysaccharides, flavonoids and terpenoids. In order to study the genetic basis of the synthesis of these active substances in the secondary metabolism of Okra, 23 026 500 short reading sequences were obtained by Illumina Hi Seq 2500 high throughput transcriptome sequencing. The average length of 42,484 Unigene, was 877 bp.31 931 and 21,926 Unigene respectively in Nr and Swiss Prot databases. Unigene was compared with COG,KOG database, which was divided into 24 classes and 25 classes according to their functions. The annotated 20 031 Unigene belong to 7 277 protein domains, and 18 602 Unigene are divided into 51 functional groups of cell components, molecular functions and biological processes, among which a large number of Unigene and cell parts, cells, and catalytic activity. According to the KEGG database, 7 619 Unigene can be located into 119 branches of metabolic pathway, of which there are 1 ~ (3) ~ (3) ~ (35) Unigene and 70 Unigene belong to anthocyanin, flavonoid, N- polysaccharide, respectively. Diterpenoids and terpenoids skeleton biosynthesis pathways that may be involved in the biosynthesis of these active substances. The results laid a foundation for the further study of anthocyanins, flavonoids and related functional genes.
【作者单位】：福建省农业科学院亚热带农业研究所;福建省农业科学院作物研究所;
【基金】：福建省公益类科研院所专项(2016R1012-4) 福建省农业科学院青年英才计划项目(YC2015-19);福建省农业科学院科技创新团队PI项目(2016PI-2)
【分类号】：S649

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