水曲柳FmABI5基因非生物胁迫及信号诱导的表达模式分析
发布时间:2018-10-20 07:12
【摘要】:ABI5(abscisic acid-insensitive 5)蛋白是一个响应ABA信号的碱性亮氨酸拉链类(basic leucine zipper,b ZIP)转录因子。揭示了水曲柳FmABI5在非生物胁迫下的表达特征,为该基因在水曲柳中代谢调控功能的研究奠定基础。获得了水曲柳ABI5基因的全长,命名为FmABI5,应用生物信息学软件分析了水曲柳FmABI5基因的分子结构特征,利用PEG、低温(4℃)及盐(NaCl)进行非生物胁迫处理,利用脱落酸(ABA)和赤霉素(GA3)进行信号诱导,分析FmABI5的表达特征。生物信息学分析表明该基因全长1 455 bp,含有完整的开放阅读框,编码484个氨基酸。FmABI5为不稳定类亲水性蛋白,不存在信号肽,具有跨膜能力,α-螺旋、延伸链、无规则卷曲分布于整个蛋白。分子进化分析结果表明,水曲柳FmABI5基因与芝麻的遗传距离较近,说明其亲缘关系较近;与马铃薯、潘那利番茄、水茄与甜椒的遗传距离较远,说明其亲缘关系较远。非生物胁迫结果表明,FmABI5基因表达水平随非生物胁迫处理时间不同而上下波动,但在处理6、48和72 h后,FmABI5基因对3种非生物胁迫处理都上调表达,说明FmABI5基因对非生物胁迫具有响应。信号诱导结果表明,外源的ABA与GA共同调节了FmABI5基因的表达。
[Abstract]:ABI5 (abscisic acid-insensitive 5) protein is a basic leucine zipper (basic leucine zipper,b ZIP transcription factor that responds to ABA signal. The expression characteristics of FmABI5 in Fraxinus mandshurica under abiotic stress were revealed, which laid a foundation for the study of metabolic regulation of the gene in Fraxinus mandshurica. The full length of ABI5 gene of Fraxinus mandshurica was obtained. The molecular structure of FmABI5 gene of Fraxinus mandshurica was analyzed by using bioinformatics software FmABI5, and abiotic stress was carried out by using PEG, low temperature (4 鈩,
本文编号:2282430
[Abstract]:ABI5 (abscisic acid-insensitive 5) protein is a basic leucine zipper (basic leucine zipper,b ZIP transcription factor that responds to ABA signal. The expression characteristics of FmABI5 in Fraxinus mandshurica under abiotic stress were revealed, which laid a foundation for the study of metabolic regulation of the gene in Fraxinus mandshurica. The full length of ABI5 gene of Fraxinus mandshurica was obtained. The molecular structure of FmABI5 gene of Fraxinus mandshurica was analyzed by using bioinformatics software FmABI5, and abiotic stress was carried out by using PEG, low temperature (4 鈩,
本文编号:2282430
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