利用高密度SNP遗传图谱定位小麦穗部性状基因

发布时间：2018-10-22 08:07

【摘要】：小麦穗部性状之间相关性密切,其中穗粒数和千粒重是重要的产量构成要素,挖掘与穗部性状相关联的基因位点对分子标记辅助育种及解释基因效应具有重要意义。本研究以RIL群体(山农01-35×藁城9411)173个F_(8:9)株系为材料,利用90 k小麦SNP基因芯片、DAr T芯片技术及传统的分子标记技术构建的高密度遗传图谱,在5个环境下进行穗部相关性状QTL定位。检测到位于1B、4B、5B、6A染色体上7个控制千粒重的加性QTL,解释表型变异率6.00%~36.30%,加性效应均来自大粒母本山农01-35;检测到8个控制穗长的加性QTL,解释表型变异率14.34%~25.44%;3个控制穗粒数的加性QTL;5个控制可育小穗数的加性QTL;3个控制不育小穗数的加性QTL,贡献率为8.70%~37.70%;4个控制总小穗数的加性QTL;6个控制小穗密度的加性QTL。通过基因型与环境互作分析,检测到32个加性QTL,解释表型变异率0.05%~1.05%。在4B染色体区段EX_C101685 RAC875_C27536检测到控制粒重、穗长、穗粒数、可育小穗数、不育小穗数、总小穗数的一因多效QTL,其贡献率为5.40%~37.70%,该位点在多个环境中被检测到,是稳定主效QTL。在6A染色体wPt-0959-TaGw2-CAPS区间上检测到控制粒重、总小穗数的QTL。研究结果为穗部性状的分子标记开发、基因精细定位和功能基因克隆奠定了基础。
[Abstract]:There is a close correlation among panicle traits, among which the number of grains per ear and 1000-grain weight are important components of yield. It is important to excavate the gene loci associated with panicle traits for molecular marker-assisted breeding and to explain gene effects. In this study, 173 F _ (8:9) lines of RIL population (Shannong 01-35 脳 Gaocheng 9411) were used as materials, the high-density genetic map was constructed by using 90k wheat SNP gene chip, DAr T chip technology and traditional molecular marker technology. QTL mapping of panicle related traits was carried out in 5 environments. An additive QTL, interpretation phenotype variation rate of 6.00 / 36.30g was detected on chromosome 1BX 4BX 5BN 6A to control 1000-grain weight, and the additive effect was all from Shannong 01-35, a large grain parent. Eight additive QTL, interpretations of phenotypic variation rates were detected, which controlled panicle length at 14.34% and 25.44%, respectively, and 3 controlled spikelets were controlled by grain number. The additive QTL, contribution rate of the male sterile spikelets was 8.70% and 37.70% respectively, and the additive QTL; of 4 total spikelets controlled the total spikelet number, and the additive QTL. of the spikelet density controlled 6% of the total spikelet number of male sterile spikelets, and the additive QTL, contribution rate of the sterile spikelet number was 8.70% and 37.70% respectively. By genotypic and environmental interaction analysis, 32 additive QTL, were detected to explain the phenotypic variation rate of 0.05% or 1.05%. In chromosome 4B, EX_C101685 RAC875_C27536 detected the controlled grain weight, panicle length, grain number per spike, fertile spikelet number, sterile spikelet number, total spikelet number and its contribution rate was 5.40% 37.70%. This locus was detected in many environments and was a stable principal QTL.. QTL. with controlled grain weight and total spikelet number was detected in the wPt-0959-TaGw2-CAPS interval of chromosome 6A. The results laid a foundation for molecular marker development, gene fine mapping and functional gene cloning of panicle traits.
【作者单位】：山东农业大学农学院小麦品质育种研究室/作物生物学国家重点实验室;
【基金】：山东省自然科学基金项目(2015ZRB01179,ZR2013CM004) 山东省种质资源创制课题资助~~
【分类号】：S512.1

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