牦牛源巴氏杆菌对氟喹诺酮类药物的耐药表型及耐药基因检测

发布时间：2018-10-22 19:07

【摘要】：从西藏不同地区规模化养殖场和兽防站(局)采集的疑似呼吸系统疾病症状的病死牦牛肺组织样品220份,通过细菌培养特性、染色镜检、微生物鉴定系统分离出44株巴氏杆菌。采用微量肉汤稀释法,测定了氟喹诺酮类6种抗菌药物和黏菌素药物对44株细菌的抗菌活性。采用PCR技术,进行氟喹诺酮类耐药基因的检测。结果表明,氟喹诺酮类6个药物的最小抑菌浓度(MIC)范围多在0.125~128μg/mL之间,黏菌素药物的MIC范围为0.016~8μg/mL。环丙沙星和加替沙星对临床分离的巴氏杆菌的耐药率分别为52.27%和54.55%,黏菌素对巴氏杆菌的耐药率为4.55%,粘菌素对大多数巴氏杆菌菌株的MIC值2μg/mL。PCR结果显示,氟喹诺酮类qnrA、qnrB、qnrS、aac(6′)-Ib-cr基因的阳性检出率分别为29.55%、18.18%、22.73%和18.18%,但qnrC、qnrD、qepA基因未检出。研究结果提示,大多数菌株对氟喹诺酮类药物存在交叉耐药现象,故临床应加强耐药性及耐药基因的检测。
[Abstract]:Forty-four strains of Pasteurella pasteuris were isolated from the lung tissue samples of infected yaks with suspected respiratory disease symptoms collected from large-scale breeding farms and veterinary prevention stations in different areas of Tibet. 44 strains of Pasteurella spp were isolated by bacterial culture, staining and microbiological identification system. The antimicrobial activities of 6 fluoroquinolones and myxin against 44 strains of bacteria were determined by broth dilution method. Fluoroquinolones resistance genes were detected by PCR technique. The results showed that the minimum inhibitory concentration (MIC) of fluoroquinolones was in the range of 0.125 ~ 128 渭 g/mL, and the MIC range of myxin was 0.016 ~ 8 渭 g 路mL ~ (-1). The resistance rates of ciprofloxacin and gatifloxacin to clinical isolates of Pasteurella were 52.27% and 54.55, respectively. The resistance rate of myxin to Pasteurella was 4.55. The MIC value of myxin to most strains of Pasteurella was 2 渭 g/mL.PCR. The positive rate of fluoroquinolones qnrA,qnrB,qnrS,aac (6')-Ib-cr gene was 29.55% and 18.18%, respectively, but qnrC,qnrD,qepA gene was not detected. The results suggested that most strains had cross resistance to fluoroquinolones, so the detection of drug resistance and resistance genes should be strengthened in clinic.
【作者单位】：西藏职业技术学院动物科学技术学院;河南农业大学牧医工程学院;
【基金】：西藏自治区高校青年教师创新支持计划项目(QCZ2016-88和QC2015-77) 西藏自治区自然科学基金项目(2016ZR-15-62和2016ZR-ZX-01)
【分类号】：S858.23

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