小桐子转录因子JcZAT10基因的分离与低温表达分析

发布时间：2018-10-23 10:31

【摘要】：利用RT-PCR技术克隆小桐子C2H2型锌指蛋白ZAT10转录因子基因c DNA序列,命名为Jc ZAT10,并对其基因结构、功能结构域、系统进化及差异表达进行了分析。结果表明,小桐子Jc ZAT10基因开放阅读框全长753bp,编码250个氨基酸,推测蛋白分子量为26.7k Da,理论等电点为8.45,并鉴定到两个锌指结构域、核定位信号序列以及富Leu保守基序。系统进化分析显示,Jc ZAT10与同属大戟科的蓖麻亲缘关系最近,序列一致性为79.6%。qRT-PCR分析表明,小桐子Jc ZAT10基因存在组织表达特异性,在茎中表达量较高,而在叶片中表达量较低,但在叶片与根中受低温诱导表达,且都在低温胁迫3h时达到最大表达量。本研究为进一步对Jc ZAT10基因进行功能验证以及其在低温信号转导中的机制研究提供了依据。
[Abstract]:The c DNA sequence of ZAT10 transcription factor gene of C2H2 type zinc finger protein was cloned by RT-PCR, named Jc ZAT10, and its gene structure, functional domain, phylogeny and differential expression were analyzed. The results showed that the open reading frame of Jc ZAT10 gene was 753 BP in length and encoded 250 amino acids. The deduced molecular weight of the protein was 26.7k Da, and the theoretical isoelectric point was 8.45. Two zinc-finger domains, nuclear localization signal sequences and conserved Leu conserved motifs were identified. Phylogenetic analysis showed that Jc ZAT10 had the closest relationship with castor (Euphorbiaceae), and the sequence consistency was 79.6%.qRT-PCR analysis. The Jc ZAT10 gene of Paulownia chinensis had tissue expression specificity, higher expression in stem and lower expression in leaves. However, the expression was induced by low temperature in leaves and roots, and reached the maximum at 3h of low temperature stress. This study provides a basis for further functional verification of Jc ZAT10 gene and its mechanism in hypothermia signal transduction.
【作者单位】：曲靖师范学院云南高原生物资源保护与利用研究中心;曲靖师范学院云南省高校云贵高原动植物遗传多样性及生态适应性重点实验室;云南师范大学生命科学学院;
【基金】：国家自然科学基金(31460179,31260064) 云南省高校科技创新团队支持计划“云南省高校云南特境内生菌资源的开发与利用科技创新团队”
【分类号】：S794.9

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