根癌农杆菌介导GUS基因转化玉米芽尖生长点的研究
发布时间：2018-10-23 16:55
【摘要】：本试验以玉米(Zea mays)杂交种‘郑单958’的芽尖生长点作为农杆菌侵染受体,以β-葡萄糖醛酸苷酶基因(GUS)作为转化基因,吸水链霉菌草丁膦乙酰转移酶基因(bar)作为筛选标记基因,通过GUS颜色反应,分别从侵染液浓度、侵染时间和真空处理时间三个方面分析了影响GUS基因转化率的因素,初步得出结论:当侵染液浓度(OD600)为0.6、侵染时间2 h、真空处理时间为10 min时,选用杂交种‘郑单958’作为受体材料将会达到较高的遗传转化率。
[Abstract]:In this experiment, the growth point of shoot tip of maize (Zea mays) hybrid 'Zhengdan 958' was used as the infection receptor of Agrobacterium tumefaciens, the 尾 -glucuronidase gene (GUS) was used as the transformation gene, and the (bar) gene of Streptomyces hygroscopicus was used as the screening marker gene. By GUS color reaction, the factors affecting the conversion of GUS gene were analyzed from three aspects: the concentration of infection solution, the time of infection and the time of vacuum treatment. It is concluded that when the concentration of infection solution (OD600) is 0.6, the infection time is 2 h, and the vacuum treatment time is 10 min, the genetic transformation rate of the hybrid 'Zhengdan 958' will reach a higher genetic transformation rate when it is selected as the recipient material.
【作者单位】： 河南农业大学农学院/河南粮食作物协同创新中心;重庆市农业科学院;
【基金】：国家自然科学基金(31071431)~~
【分类号】：S513
本文编号：2289893
[Abstract]:In this experiment, the growth point of shoot tip of maize (Zea mays) hybrid 'Zhengdan 958' was used as the infection receptor of Agrobacterium tumefaciens, the 尾 -glucuronidase gene (GUS) was used as the transformation gene, and the (bar) gene of Streptomyces hygroscopicus was used as the screening marker gene. By GUS color reaction, the factors affecting the conversion of GUS gene were analyzed from three aspects: the concentration of infection solution, the time of infection and the time of vacuum treatment. It is concluded that when the concentration of infection solution (OD600) is 0.6, the infection time is 2 h, and the vacuum treatment time is 10 min, the genetic transformation rate of the hybrid 'Zhengdan 958' will reach a higher genetic transformation rate when it is selected as the recipient material.
【作者单位】： 河南农业大学农学院/河南粮食作物协同创新中心;重庆市农业科学院;
【基金】：国家自然科学基金(31071431)~~
【分类号】：S513
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