中华蜜蜂6日龄幼虫肠道响应球囊菌胁迫的差异表达基因分析

发布时间：2018-10-24 15:35

【摘要】：蜜蜂球囊菌特异性侵染蜜蜂幼虫而导致白垩病,严重危害养蜂生产。本研究利用RNA-seq技术对健康及球囊菌胁迫的中蜂幼虫肠道进行深度测序,进而对宿主的差异表达基因进行深入分析。本研究中,幼虫肠道样品的RNA-seq共得到191167730条原始读段(raw reads),经过滤得到186284296条有效读段(clean reads),差异表达基因(DEG)分析结果显示上调与下调基因的数量分别为4513和385个。Gene ontology(GO)富集分析结果显示,上调基因富集在45个GO条目(term),富集基因数最多的是细胞进程、代谢进程及催化活性,下调基因富集在32个GO term,富集基因数最多的是代谢进程、单组织进程及催化活性,KEGG代谢通路(pathway)富集分析结果显示上调基因富集在193个pathway,其中富集基因数最多的是核糖体、氨基酸的合成、碳代谢。下调基因富集在59个pathway,其中富集基因数最多的是甘氨酸、碳代谢以及二羧酸代谢。深入分析发现宿主的细胞免疫被显著激活,体液免疫中的Toll-like与Jak-STAT信号通路也被球囊菌所激活。研究结果为揭示中蜂幼虫在球囊菌入侵后期的胁迫应答机制提供了重要的信息,也为解析中蜂幼虫的球囊菌抗性机制奠定了基础。
[Abstract]:Bee Balloon bacteria infects bee larvae and results in chalkiness, which seriously harms beekeeping production. In this study, RNA-seq technique was used to analyze the differentially expressed genes of the host. In this study, A total of 191167730 (raw reads), fragments were obtained from RNA-seq of larval intestinal samples. 186284296 effective (clean reads), differentially expressed genes were obtained by filtration. The results of (DEG) analysis showed that the number of up-regulated and down-regulated genes was 4513 and 385. Gene ontology (GO) enrichment analysis, respectively, and the results showed that the number of up-regulated and down-regulated genes was 4513 and 385. Gene ontology (GO) enrichment analysis, respectively. The up-regulated gene enrichment in 45 GO entries (term), enrichment gene number is the most cell process, metabolic process and catalytic activity, down-regulated gene enrichment in 32 GO term, enrichment gene number is the most metabolic process. Single tissue process and catalytic activity. (pathway) enrichment analysis of KEGG metabolic pathway showed that up-regulated genes were enriched in 193 pathway,. The most abundant genes were ribosome, amino acid synthesis and carbon metabolism. The down-regulated genes were enriched in 59 pathway, and the most abundant genes were glycine, carbon metabolism and dicarboxylic acid metabolism. Further analysis showed that the cellular immunity of the host was significantly activated, and the Toll-like and Jak-STAT signaling pathways in humoral immunity were also activated by the balloon bacteria. The results provided important information for revealing the stress response mechanism of Chinese wasp larvae in the late stage of the Balloon invasion, and also laid a foundation for the analysis of the Balloon resistance mechanism of Chinese wasp larvae.
【作者单位】：福建农林大学蜂学学院;新疆维吾尔自治区蜂业技术管理总站;
【基金】：现代农业产业技术体系建设专项资金(CARS-45-KXJ7) 福建农林大学科技发展资金(KF2015123)
【分类号】：S895

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