蚬壳花椒GID1同源基因克隆及其在种子萌发过程中的表达分析

发布时间：2018-10-25 09:48

【摘要】：GID1是赤霉素信号通路关键调控基因,其通路主要影响种子萌发和植物生长。本文以高通量测序构建的蚬壳花椒(Zanthoxylum dissitum)cDNA文库为参考,克隆了蚬壳花椒3个GID1同源基因ZaGID1a、ZaGID1b、ZaGID1c。利用生物信息学对蚬壳花椒GID1同源基因的核酸序列及氨基酸序列进行分析,表明3个同源GID1序列编码的蛋白功能区域、理化性质、系统发育、亲疏水性、亚细胞信号肽定位、三级结构预测等各项生物信息学信息均指向赤霉素受体蛋白功能。同源GID1序列在蚬壳花椒种子人工萌发过程中的实时荧光定量PCR(qRT-PCR)表达量分析研究表明,ZaGID1a、ZaGID1c可能对蚬壳花椒种子萌发起关键调控作用,ZaGID1b与种子萌发过程的关系不明显。
[Abstract]:GID1 is a key regulation gene of gibberellin signaling pathway which mainly affects seed germination and plant growth. Based on the (Zanthoxylum dissitum) cDNA library constructed by high throughput sequencing, three GID1 homologous genes ZaGID1a,ZaGID1b,ZaGID1c. were cloned. The nucleic acid sequence and amino acid sequence of GID1 homologous gene in Shell pepper were analyzed by bioinformatics. The results showed that three homologous GID1 sequences encoded protein functional region, physical and chemical properties, phylogeny, hydrophobicity, subcellular signal peptide localization. Bioinformatics information, such as tertiary structure prediction, all point to the function of gibberellin receptor protein. The analysis of real-time fluorescence quantitative PCR (qRT-PCR) expression of homologous GID1 sequence in the process of seed germination showed that ZaGID1a,ZaGID1c may play a key role in the seed germination, but the relationship between ZaGID1b and seed germination was not obvious.
【作者单位】：中南林业科技大学生命科学与技术学院;中南林业科技大学环境科学与工程学院;
【基金】：国家自然科学基金(31370612) 湖南省高校科技成果产业化繁育项目(15CY011)~~
【分类号】：S567.19

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