刀鲚嗅觉受体基因MOR-51I2克

发布时间：2018-11-03 16:19

【摘要】：为研究刀鲚的嗅觉受体(Olfactory receptor,OR)是否参与其生殖洄游过程,利用基因组步移技术,从洄游型刀鲚(Coilia nasus)中克隆出嗅觉受体基因MOR-51I2的基因序列全长。该基因为单外显子结构,编码区长为999 bp。在3′UTR区域具有一段微卫星序列,以(AC)n为重复单位,并夹有若干T或G碱基,且在不同生态型间具有明显长度差异。MOR-51I2基因所编码的蛋白具有7次疏水性α-螺旋的跨膜结构,为G-蛋白偶联受体。MOR-51I2基因与已报道的其他鱼类的OR基因所编码的氨基酸序列的同源性在51%以上,其中与大西洋鲱(Clupea harengus)的OR51I2-like基因的同源性高达83%。经q RT-PCR分析显示,在定居型刀鲚中,MOR-51I2基因主要在嗅囊和性腺中表达,在肝脏、鳃、肌肉微弱表达,在心脏和眼睛中几乎不表达。其中,雌性嗅囊中的表达量约是雄性嗅囊中的2倍,是精巢和卵巢中的80—100倍。在洄游型刀鲚中,该基因在雄性嗅囊的表达量约是雌性嗅囊中的6倍。MOR-51I2基因在洄游型刀鲚的雌性嗅囊中的表达量约是定居型刀鲚的雌性嗅囊中的1/5,而在洄游型刀鲚的雄性嗅囊中的表达量却是定居型刀鲚的雄性嗅囊中的3倍。这些结果表明,MOR-51I2基因不但参与刀鲚的嗅觉功能,而且可能参与了刀鲚的性腺发育及生殖洄游过程,同时也可能与其生态型的分化相关。
[Abstract]:In order to study whether the olfactory receptor (Olfactory receptor,OR) of Coilia ectenes is involved in the reproductive migration process, the full length of the olfactory receptor gene MOR-51I2 gene was cloned from the (Coilia nasus) of Coilia ectenes by genomic step technique. The gene has a single exon structure and a coding region of 999 bp.. There is a microsatellite sequence in the 3'UTR region, repeated in (AC) n, with a number of T or G bases, There were significant length differences among different ecotypes. The protein encoded by MOR-51I2 gene had a transmembrane structure of seven times hydrophobic 伪 -helix. The amino acid sequence of MOR-51I2 gene is more than 51% homology with OR gene of other reported fish, and the homology with OR51I2-like gene of Atlantic herring (Clupea harengus) is as high as 83%. Q RT-PCR analysis showed that MOR-51I2 gene was mainly expressed in olfactory sac and gonad in Coilia ectenes, weakly expressed in liver, Gill and muscle, and hardly expressed in heart and eyes. The expression level in female olfactory sac was about twice as high as in male olfactory sac and 80-100 times higher than that in testis and ovaries. The expression of the MOR-51I2 gene in the female olfactory sac of the migratory Coilia is about 6 times higher than that in the female olfactory sac. The expression of the MOR-51I2 gene in the female olfactory sac of the migratory Coilia is about 1 / 5 of the female olfactory sac of the settled Coilia. The expression level in the male olfactory sac of Coilia ecilia is 3 times higher than that of the settled Coilia ecilia. These results suggest that the MOR-51I2 gene is involved not only in the olfactory function of Coilia coilia, but also in the gonadal development and reproductive migration of Coilia ectenes, and may also be related to its ecotype differentiation.
【作者单位】：上海海洋大学鱼类研究室;上海市海洋动物系统分类与进化重点实验室;
【基金】：公益性行业(农业)科研专项(201203065) 国家自然科学基金(31172407,31472280) 高等学校博士学科点科研专项(20123104110006) 上海高校水产学高峰学科资助~~
【分类号】：S917.4

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