ADMA代谢酶基因多态性增加个体罹患心脑血管疾病风险

发布时间：2018-11-08 19:35

【摘要】：为了探讨ADMA代谢酶基因多态性对个体罹患心脑血管疾病的机制,收集我院2012年~2016年经冠脉造影确诊的冠心病患者742例(试验组),同时收集我院体检中心的975例健康志愿者(对照组)。采集静脉血提取基因组DNA,采用PCR和限制性内切酶法检测DDAH1的基因位点多态性和酶联免疫法测定ADMA浓度。采用SPSS15.0软件进行数据统计分析,结果发现,试验组C144563T位点CC基因型频率及C等位基因频率均要明显高于对照组,其差异有统计学意义(p0.05)。logistic回归分析结果显示,DDAH1基因CC基因型或144563C等位的个体患CHD的风险显著增加(OR=1.752,p=0.007)。试验组C~(143611)G位点GC基因型频率及C等位基因频率均要明显高于对照组,其差异有统计学意义(p0.05)。logistic回归分析结果显示,DDAH1基因GC基因型或143611C等位的个体患CHD的风险显著增加(OR=1.890,p=0.004)。试验组人群血浆ADMA平均浓度为(3.07±0.51)μmol/mL,明显高于对照组人群血浆ADMA平均浓度(1.83±0.39)μmol/mL,差异有统计学意义(p=0.0030.05)。C~(144563)T位点CC基因型与C~(143611)G位点GC基因型试验组个体血浆ADMA平均浓度((3.09±0.47)μmol/mL,(3.21±0.54)μmol/mL)均要显著高于对照组ADMA平均浓度((1.96±0.40)μmol/mL,(1.89±0.41)μmol/mL),其差异均有统计学意义(p=0.021,0.0140.05)。我们推论且DDAH1基因的C~(144563)T位点与C~(143611)G位点与CHD密切相关,其影响机制可能是通过C~(144563)T位点与C~(143611)G位点基因型的改变而增高ADMA水平,从而导致CHD的发生与发展。
[Abstract]:In order to investigate the mechanism of ADMA metabolic enzyme gene polymorphism on cardiovascular and cerebrovascular diseases in individuals, 742 patients with coronary heart disease diagnosed by coronary angiography in our hospital from 2012 to 2016 (trial group) were collected. At the same time, 975 healthy volunteers (control group) were collected from the physical examination center of our hospital. Genomic DNA, was extracted from venous blood. PCR and restriction endonuclease method were used to detect the polymorphism of DDAH1 gene locus and the concentration of ADMA by enzyme linked immunosorbent assay (Elisa). The data were statistically analyzed by SPSS15.0 software. The results showed that the CC genotype frequency and C allele frequency of C144563T locus in the trial group were significantly higher than those in the control group, and the difference was statistically significant (p0.05). Logistic regression analysis showed that the frequency of C144563T locus and C allele was significantly higher than that of the control group. Individuals with DDAH1 CC genotype or 144563 C allele had a significantly increased risk of CHD (OR=1.752,p=0.007). The frequency of GC genotype and C allele at C ~ (143611) G locus in the trial group was significantly higher than that in the control group, and the difference was statistically significant (p0.05). Logistic regression analysis showed that the frequency of C ~ (143611) G locus GC genotype and C allele was significantly higher than that of the control group. Individuals with GC genotype or 143611 C allele of DDAH1 gene had a significantly increased risk of CHD (OR=1.890,p=0.004). The average concentration of plasma ADMA in the trial group was (3.07 卤0.51) 渭 mol/mL, significantly higher than that in the control group (1.83 卤0.39) 渭 mol/mL,. The average plasma ADMA concentration of C ~ (144563) T locus CC genotype and C ~ (143611) G GC genotype group was (3.09 卤0.47) 渭 mol/mL,. (3.21 卤0.54) 渭 mol/mL was significantly higher than that of the control group (1.96 卤0.40) 渭 mol/mL, (1.89 卤0.41) 渭 mol/mL, the difference was statistically significant (p0.05). We infer that the C144563 T locus of DDAH1 gene is closely related to C143611 G locus and CHD, and the mechanism may be that the ADMA level is increased by the genotype change of C144563 T locus and C143611 G locus. This leads to the occurrence and development of CHD.
【作者单位】：重庆市中医院;
【基金】：重庆市中医院资助
【分类号】：R541.4

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