拟南芥HY2基因在NaCl信号转导途径中的功能研究
发布时间：2018-11-09 10:29
【摘要】：近些年来,全球土壤盐渍化问题日益严重,影响了世界农业的生产与发展。盐胁迫作为一个主要的非生物胁迫,严重影响了植物的生长发育。高盐浓度导致植物离子稳态失衡,渗透平衡紊乱,同时引起各种次级胁迫。植物则通过调控复杂的信号网络来应对盐胁迫,包括Ca2+依赖型信号转导途径和Ca2+不依赖型信号转导途径。目前,利用分子生物学技术对盐胁迫诱导基因的功能研究成为提高植物耐盐性的重要方法。本实验以含有luciferase荧光蛋白的过表达拟南芥为材料,以200m M Na Cl为筛选条件做种子萌发实验,获得了盐胁迫敏感基因HY2。HY2基因编码了一个光敏色素胆素合成酶,负责质体内光敏色素生色团的合成,并参与调控红光及远红光下的下胚轴伸长。HY2蛋白N端的45个氨基酸有叶绿体转运肽的功能,且HY2蛋白定位在质体里。后期结合突变体材料hy2从分子水平探讨HY2基因在Na Cl信号转导途径下的功能。主要研究结果如下:(1)HY2基因在拟南芥中的表达无组织特异性,在根部表达量较低,花中表达量较高;(2)HY2基因在转录水平受盐胁迫诱导,且hy2突变体表现为对盐不敏感,表明HY2可能是植物盐胁迫信号通路中的一个负调控因子;HY2基因在蛋白水平不受盐胁迫诱导;(3)盐胁迫应答基因的表达分析结果表明,HY2通过不依赖ABA的方式来响应盐胁迫。同时在盐胁迫下,hy2突变体能抑制DREB2A、KIN2等基因的表达;(4)相对于Col4生态型,盐胁迫对红光下hy2突变体下胚轴伸长的抑制程度较强;(5)在生理生化水平,hy2突变体可以通过增加脯氨酸的积累,减少叶绿素的损失来提高植物的耐盐性。
[Abstract]:In recent years, the problem of soil salinization in the world is becoming more and more serious, which affects the production and development of world agriculture. Salt stress, as a major abiotic stress, seriously affects the growth and development of plants. High salt concentration leads to imbalance of plant ion homeostasis, disturbance of osmotic balance and various secondary stresses. Plants respond to salt stress by regulating complex signal networks, including Ca2 dependent signal transduction pathways and Ca2 independent signal transduction pathways. At present, the study of the function of salt stress inducing genes by molecular biology has become an important method to improve the salt tolerance of plants. In this experiment, the overexpression Arabidopsis thaliana containing luciferase fluorescent protein was used as the material, and 200m M Na Cl was used as the screening condition for seed germination experiment. The salt stress sensitive gene HY2.HY2 gene encoded a Guang Min pigment choline synthase. Guang Min was responsible for the synthesis of pigment chromophore and involved in regulating the elongation of hypocotyls under red and far red light. The 45 amino acids at the N-terminal of HY2 protein had the function of chloroplast transporter peptide and HY2 protein was located in the plastids. The function of HY2 gene in Na Cl signal transduction pathway was studied at the molecular level with the mutant hy2 at late stage. The main results are as follows: (1) the expression of HY2 gene in Arabidopsis thaliana has no tissue specificity and is low in root and high in flower; (2) HY2 gene was induced by salt stress at the transcription level, and hy2 mutant was not sensitive to salt, suggesting that HY2 may be a negative regulator in plant salt stress signaling pathway, and HY2 gene is not induced by salt stress at protein level. (3) the results of salt stress response gene analysis showed that HY2 responded to salt stress by ABA independent. At the same time, hy2 mutant could inhibit the expression of DREB2A,KIN2 and other genes under salt stress. (4) compared with Col4 ecotype, salt stress inhibited the elongation of Hypocotyl of hy2 mutant under red light. (5) at the physiological and biochemical level, hy2 mutants can improve plant salt tolerance by increasing proline accumulation and reducing chlorophyll loss.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：Q943.2
本文编号：2320143
[Abstract]:In recent years, the problem of soil salinization in the world is becoming more and more serious, which affects the production and development of world agriculture. Salt stress, as a major abiotic stress, seriously affects the growth and development of plants. High salt concentration leads to imbalance of plant ion homeostasis, disturbance of osmotic balance and various secondary stresses. Plants respond to salt stress by regulating complex signal networks, including Ca2 dependent signal transduction pathways and Ca2 independent signal transduction pathways. At present, the study of the function of salt stress inducing genes by molecular biology has become an important method to improve the salt tolerance of plants. In this experiment, the overexpression Arabidopsis thaliana containing luciferase fluorescent protein was used as the material, and 200m M Na Cl was used as the screening condition for seed germination experiment. The salt stress sensitive gene HY2.HY2 gene encoded a Guang Min pigment choline synthase. Guang Min was responsible for the synthesis of pigment chromophore and involved in regulating the elongation of hypocotyls under red and far red light. The 45 amino acids at the N-terminal of HY2 protein had the function of chloroplast transporter peptide and HY2 protein was located in the plastids. The function of HY2 gene in Na Cl signal transduction pathway was studied at the molecular level with the mutant hy2 at late stage. The main results are as follows: (1) the expression of HY2 gene in Arabidopsis thaliana has no tissue specificity and is low in root and high in flower; (2) HY2 gene was induced by salt stress at the transcription level, and hy2 mutant was not sensitive to salt, suggesting that HY2 may be a negative regulator in plant salt stress signaling pathway, and HY2 gene is not induced by salt stress at protein level. (3) the results of salt stress response gene analysis showed that HY2 responded to salt stress by ABA independent. At the same time, hy2 mutant could inhibit the expression of DREB2A,KIN2 and other genes under salt stress. (4) compared with Col4 ecotype, salt stress inhibited the elongation of Hypocotyl of hy2 mutant under red light. (5) at the physiological and biochemical level, hy2 mutants can improve plant salt tolerance by increasing proline accumulation and reducing chlorophyll loss.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：Q943.2
【相似文献】
相关硕士学位论文 前2条
1 张梦龙;拟南芥HY2基因在NaCl信号转导途径中的功能研究[D];吉林大学;2016年
2 蒋宇;HY2基因参与调控铝抑制拟南芥根伸长作用机制研究[D];吉林大学;2014年
，本文编号：2320143
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2320143.html
最近更新
教材专著
