刺参不同组织中MnSOD基因在脂多糖刺激下的定量表达分析（英文）

发布时间：2018-11-10 23:33

【摘要】：刺参由于其重要的经济价值和药用价值,已经成为我国海水养殖的重要种类。MnSOD对于增强吞噬细胞防御能力和整个机体的免疫功能具有重要作用,与生物体抵御病毒细菌等能力密切相关。通过研究在脂多糖刺激下MnSOD基因的定量表达情况,分析MnSOD在刺参免疫系统中的作用。结果显示:MnSOD基因的表达量由肠、肌肉到管足逐渐降低,体壁的表达量与体腔液基本一致。当刺参暴露于脂多糖中时,除肠以外其他的四种组织中MnSOD基因均出现过表达。其中,体腔液和体壁组织中MnSOD基因的表达量于12 h达到峰值;肌肉和管足中MnSOD基因的表达量于48 h达到峰值;体腔液中MnSOD基因的表达量于12 h达到对照组的4 115倍,而肠组织中MnSOD基因的表达量在48 h的实验过程中呈波动性降低。总体上来说,在脂多糖刺激下刺参各组织中MnSOD基因的表达量呈规律性波动。实验结果表明:在脂多糖刺激下刺参个体会产生强烈的抗氧化反应以保护刺参免受病原微生物的感染。
[Abstract]:Because of its important economic value and medicinal value, sea cucumber has become an important species of marine culture in China. MnSOD plays an important role in enhancing phagocyte defense and immune function of the whole body. It is closely related to the ability of organism to resist virus and bacteria. The role of MnSOD in the immune system of sea cucumber was analyzed by studying the quantitative expression of MnSOD gene stimulated by lipopolysaccharide. The results showed that the expression of MnSOD gene decreased gradually from intestine, muscle to tubula, and the expression of MnSOD gene in body wall was basically consistent with that in body cavity fluid. When Acanthopsis japonicus was exposed to lipopolysaccharide, MnSOD gene was expressed in all four tissues except intestine. The expression of MnSOD gene in body cavity fluid and body wall reached the peak at 12 h, and the expression of MnSOD gene in muscle and tube foot reached the peak at 48 h. The expression of MnSOD gene in somatic cavity fluid was 4 115 times higher than that in control group at 12 h, while the expression of MnSOD gene in intestinal tissue decreased at 48 h. In general, the expression of MnSOD gene fluctuated regularly in the tissues stimulated by lipopolysaccharide. The results showed that the individuals of Acanthopsis japonicus were stimulated by lipopolysaccharide to produce strong antioxidant reactions to protect them from pathogenic microorganisms.
【作者单位】： 大连海洋大学农业部北方海水增养殖重点实验室;
【基金】：Supported by grants from department of education Liaoning province(L201620) National nature science foundation of China(41106128)
【分类号】：S917.4

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