Advance in Application of Transgenic Breeding Technology in
本文关键词:转基因育种技术在菊花性状改良中的应用进展,由笔耕文化传播整理发布。
转基因育种 菊花性状改良
园 艺 学 报 2013,40(9):1813–1825 http: // ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@ 转基因育种技术在菊花性状改良中的应用进展 成丽娜,魏 倩,Muhammad Imtiaz,高俊平,洪 波*
(中国农业大学观赏园艺与园林系,北京 100193)
摘 要:自1989年发现了菊花对农杆菌浸染的敏感性,农杆菌介导转化法已成功应用于菊花花色、
花期、株形、抗病虫及耐非生物胁迫等性状的改良中。从影响菊花遗传转化效率的农杆菌菌株类型、外
植体选用、辅助添加剂的使用以及抗生素筛选等几方面阐述了菊花遗传转化技术研究的进展和应用现状,对菊花分子育种性状改良和重要功能基因发掘等研究进展进行综述,并对菊花转化效率改善途径的探索
和环境友好型转基因新技术研发前景提出展望,以期为菊花分子育种提供参考。
关键词:菊花;农杆菌;转基因;分子育种;性状改良
中图分类号:S 682.1+1 文献标志码:A 文章编号:0513-353X(2013)09-1813-13
Advances in Application of Transgenic Breeding Technology in the Traits Improvement of Chrysanthemum
CHENG Li-na,WEI Qian,Muhammad Imtiaz,GAO Jun-ping,and HONG Bo*
(Department of Ornamental Horticulture,China Agricultural University,Beijing 100193,China)
Abstract:Following the discovery of chrysanthemum sensitivity to Agrobacterium in 1989,the technique of Agrobacterium-mediated genetic transformation has been successfully used in improvement of traits such as flower color,plant style,flowering time,,disease-insect resistance and abiotic stress tolerance. In this review,the advances of Agrobacterium-mediated genetic transformation in chrysanthemum were summarized,including Agrobacterium strains,explants for infection,the addition of chemical compounds and selection markers. Also,the improvement of traits,exploration of important genes was reviewed and the perspective of enhancing transformation efficiency and developing environment-friendly transgenic technology were discussed. The main goal is to provide reference for molecular breeding of chrysanthemum.
Key words:chrysanthemum;Agrobaterium;transgenic technology;molecular breeding;improvement of traits
通过常规的杂交育种手段,菊花[Chrysanthemum morifolium(Ramat.)Tzvel)]的品种改良已在株形、花色、花期、抵抗病虫害和非生物胁迫等方面取得了显著的成绩。但菊花高度杂合的特点使其在杂交育种中不确定性很强,生殖或地理隔离使其无法充分利用近缘种属植物的优良基因资源,常规方法育种存在较大的局限性。转基因育种目前已成为菊花新品种培育的重要手段,外源基因的导入有针对性地改善了目标性状并保留原有优良性状,实现了利用新基因资源改良菊花品种的目标。 收稿日期:2013–06–21;修回日期:2013–08–20
基金项目:北京市园林绿化局计划项目(YLHH201300104)
*通信作者 Author for correspondence(E-mail:hongbo1203@)
本文关键词:转基因育种技术在菊花性状改良中的应用进展,由笔耕文化传播整理发布。
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