transgenic maize 在 农作物 分类中 的翻译结果

  本文关键词：基因枪法获得玉米转基因植株的研究，由笔耕文化传播整理发布。

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transgenic maize

    SCMV-resistant Transgenic Maize Obtained by Using Different Strategies

    利用不同策略获得抗SCMV转基因玉米的研究

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    EXPRESSION ACTIVITY OF MAIZE Ubi-1 PROMOTER IN FERTILE TRANSGENIC MAIZE PLANTS

    玉米Ubi-1启动子在可育转基因玉米植株中的表达活性

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    The Research and Application Transgenic Maize

    转基因玉米的研究与应用

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    Effects of the Foreign Gene Encoding a Lysine Rich Protein on Protein Compositions in Transgenic Maize Grain

    外源高赖氨酸蛋白质基因对转基因玉米种子中蛋白质组分的影响

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    Specific Detection of Alien DNA Sequences in Six Transgenic Maize by Using Multiplex PCR Method

    用复合PCR方法检测6种转基因玉米中外源DNA的特异性

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    Developments in Transgenic Maize

    玉米转基因研究进展

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    Research and Developments in Transgenic Maize

    玉米转基因研究与进展

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    Study on the Development of Transgenic Maize plants by Bombardment Transformation

    基因枪法获得玉米转基因植株的研究

    In this paper, the author reviewed the research advances of transgenic maize in transfergenic receptor system,target genes and transformation methods. Further, this paper briefly introduced the questions and ways of transgenic maize and the advances on other types maize in recent years

    在此,就玉米转基因在受体系统、目的基因及转化方法方面的研究成果,尤其是近年来玉米转基因研究方面存在的问题、解决途径以及其他类型玉米的转基因研究进展做了综述。

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    In this paper,the new research and developments made in various aspects of transgenic maize was summarized,including selection of receptor,methods and theory of transformation,selection of marker genes and promoters,establishment of expression vector,aiming characters and the prospect of application.

    从玉米转基因的受体选择、遗传转化方法和原理、标记基因的选择、表达载体构建和启动子的选择及玉米转基因研究、生产和应用前景等方面,阐述了玉米转基因研究的进展。

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    The mortalities of the 1st to 3rd instar larvae feeding on Bt transgenic maize for 1～2 days were up to 90%,and no death occurred in the 4th instar larvae,but the growth decreased.

    1-3龄幼虫取食转B t基因玉米后1-2 d死亡,死亡率达90%以上; 4龄以后死亡率为0,但其生长发育均受到一定的抑制;

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    The result showed that integration of foreign gene Fe- SOD into the genome of the TO transgenic maize plants was confirmed by PCR、PCR-Southern blot and RT-PCR and the gene was expressed at the transcirption level.

    PCR、PCR-Southern、RT-PCR分子检测结果表明:Fe-SOD基因被整合到玉米基因组中,并在转录水平上表达。

    Bio-assays and tissue section examinations were conducted for determining the resistance of Bt transgenic maize N3030Bt to Ostrinia fumacalis.

    以普通玉米杂交组合N3030为对照,通过田间调查、盆栽接虫试验、室内饲养和组织切片,系统研究了转Bt基因玉米杂交组合N3030Bt对亚洲玉米螟的抗性表现。

    Cloning of a Lysine-rich Gene SBgLR and the Effect of Improving the Protein and Lysine Content in Transgenic Maize Seed

    高赖氨酸蛋白基因SBgLR的克隆及其对提高玉米种子中蛋白质和赖氨酸含量的作用

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    Production and Analysis of Transgenic Maize with Improved Salt Tolerance by the Introduction of AtNHX1 Gene

    转AtNHX1基因玉米的产生及其耐盐性分析(英文)

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  transgenic maize

Results of Southern and Western blotting analysis certified that this gene had integrated and been expressed in transgenic maize plants and their progeny.

Ten percent of the transgenic maize expressed single fusion proteins from the translational fusion gene cry1B-1Ab and showed resistance to these two pests as well as to the fall armyworm.

Transgenic maize plants that carried the cry1B gene in T1 to T3 progenies transmitted trangenes with expected Mendelian segregation and conferred resistance to the two target insects.

Expression and inheritance of the wheat Glu-1DX5 gene in transgenic maize

We have produced transgenic maize plants containing a wheat Glu-1Dx5 gene encoding the high-molecular-weight glutenin subunit 1Dx5.

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Several maize inbreds were transformed with Agrobacterium tumefaciens EHA101 (pGIH). Transgenic maize plants were obtained. Frequency of transformation of maize inbred Suyu No. 1 can reach 8. 1%. Results of PCR and Southern blot analysis proved that T-DNA was stably integrated into the genome of maize. Staining with X-gluc confirmed the expression of GUS gene in maize cells. The band amplified by inverse PCR

用带有质粒pGIH(35S-intron-GUS/ Hptr)的根癌农杆菌EHA101转化玉米愈伤组织,获得潮霉素抗性植株,再生性好的苏玉1 图7 Southern blot分析HpaII消化的质粒pGIH 和gusA基因沉默的愈伤组织的总DNA Fig.7 Southern blot analysis of plasmid pGIH(P) and plant genomic DNA of gusA gene si- lence callus(T)digested with HpaII号转化率可以达到8.1%。对转化植株进行GUS染色分析及PCR和Southern杂交检测证明外源基因已经整合,并能够稳定表达。反向PCR分析的三个转化植株,T-DNA插入片段均为单拷贝。部分失去分化能力的抗性愈伤组织,Southern blot分析发现其基因组中有gusA基因插入,但X-gluc染色呈阴性,经HpaII酶切分析发现整合的gusA基因发生了高度甲基化。

Foreign insecticidal genes were transgerred into 2 elite inbred lines of maize (Zea mays L.) by microprojectile bombardment. It was found that a series of measures could effectively reduce clonal variation and elevate the rate of viability of transgenic maize during the processes of calli receptor preparation, selection, regeneration and planting.

通过基因枪转化法将外源抗虫基因导入 1个玉米 (ZeamaysL .)优良自交系。对受体愈伤的制备 ,抗性愈伤的筛选分化和转基因植株的移栽等方面的研究 ,采用一系列的措施 ,有效地降低了转化植株的畸形率 ,提高了转化植株的成活率

s:Techniques such as Agrobacteria,particulate gun and PEG inducing techniques are often adopted in maize transgenic research,While vigorous part such as callus and yong embryo typically are used as acceptance.Transgenic Bt maize and transgenic herbicide-resistant maize are successfully developed.World wide conscious to environmental pollution protection and feed chains remains obstacle to Transgenic maize application.

玉米转基因的方法主要有农杆菌、基因枪、PEG介导等方法 ,受体主要是愈伤组织和幼胚等生活较旺盛的部位。目前在玉米上的成功应用是抗玉米螟的Bt转基因玉米、抗除草剂转基因玉米等种类。转基因技术目前面临的问题是关于环境和食物链的问题。也就未来玉米的转基因发展进行了讨论。

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  本文关键词：基因枪法获得玉米转基因植株的研究，由笔耕文化传播整理发布。