老年糖尿病患者白色念珠菌感染临床分离株的基因分型

发布时间：2018-11-12 21:20

【摘要】：目的探讨老年糖尿病患者白色念珠菌感染临床分离株的基因分型方法。方法老年糖尿病患者发生念珠菌感染的临床标本185例,采用API20CAUX酵母菌鉴定系统鉴定,提取DNA后采用PCR方法扩增白色念珠菌25S r DNA基因内含子区和特征性重复序列(RPS)对白色念珠菌进行基因分型。结果采用25S r DNA引物扩增后的产物分别为450 bp、840 bp及450和840 bp,分别代表基因型A、B、C;185例样本菌株中,51.9%为A型,29.2%为B型,18.9%为C型;采用根据ALT重复序列RPS设计引物扩增后的产物可将基因型A、B、C分为10个亚组,A型中24例为A2,50例为A3,12例为A2/3,8例为A3/4。54例B型中,15例为B2,7例为B3,25例为B2/3,7例为B3/4。35例C型中25例为C2,10例为C2/3。结论利用25S r DNA和ALT重复序列RPS设计引物扩增产物对老年糖尿病患者样本中白色念珠菌进行基因分型方法具有快速、简捷、可靠、重复性好等优点,可提高治疗效果,减少抗生素滥用和耐药性的产生。
[Abstract]:Objective to study the genotyping of clinical isolates of Candida albicans infection in elderly diabetic patients. Methods 185 clinical specimens of senile diabetic patients with Candida infection were identified by API20CAUX yeast identification system. After DNA was extracted, the intron region of 25S r DNA gene of Candida albicans was amplified by PCR method and the characteristic repeat sequence (RPS) was used to type Candida albicans. Results the products amplified by 25S r DNA primer were 450 bp,840 bp and 450 and 840 bp, respectively. Of the 185 isolates, 51.9% were type A, 29.2% were type B, and 18.9% were genotype C. According to the primers designed according to ALT repeat sequence RPS, the genotypes were divided into 10 subgroups. In A type A, 24 cases were A2N, 50 cases were A3, 12 cases were A2 / 3P, 8 cases were A3 / 3 / 4. 54 cases were type B, respectively. 15 cases were B2P 7 cases B3P 25 cases B2 / 3P 7 cases B3 / 4.35 cases C type C 25 cases C2P 10 cases C 2 / 3 3. Conclusion the method for genotyping Candida albicans in elderly diabetic patients by using 25S r DNA and ALT repeat RPS designed primers has the advantages of fast, simple, reliable and reproducible, and can improve the therapeutic effect. Reduce antibiotic abuse and drug resistance.
【作者单位】：齐齐哈尔医学院附属第二医院;
【基金】：齐齐哈尔科学技术局指令性计划项目(No.SFGG-201320)
【分类号】：R519;R587.1

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