基于太赫兹时域光谱的转基因与非转基因棉花种子鉴别

发布时间：2018-11-14 17:33

【摘要】：太赫兹光谱是介于红外与微波间的电磁波谱,很多生物大分子在该波段具有指纹特性。为探索棉花种子的转基因机理和性状表达的太赫兹光谱特性,该文利用太赫兹时域光谱技术对3种转基因棉花种子和1种常规育种棉花种子进行了鉴别试验探索研究。试验首先研究了棉花种子压片制备方法,然后借助太赫兹时域光谱设备,采集了4种棉花种子在有效频率范围内的太赫兹光谱信息,进而对太赫兹光谱包含的特征信息进行了定性分析,并初步探讨了4种样品的太赫兹吸收特性。试验结果表明:4种棉花种子在太赫兹波段呈现不同的光谱响应特性,尤其是在其吸光系数光谱图中,3种转基因棉种在1.21、1.23和1.41 THz处,对太赫兹呈现明显的吸收,而非转基因棉种却呈现微弱的吸收或不吸收;这些明显的差异表明太赫兹光谱可以作为鉴别转基因/非转基因棉花种子有效工具,该研究可为进一步揭示太赫兹光谱检测机理,发展转/非转基因物质快速检测方法提供参考。
[Abstract]:Terahertz spectrum is an electromagnetic spectrum between infrared and microwave. Many biological macromolecules have fingerprint characteristics in this band. In order to explore the transgenic mechanism of cotton seeds and the terahertz spectral characteristics of their characters, three transgenic cotton seeds and one conventional breeding cotton seed were identified by THz time domain spectroscopy. The preparation method of cotton seed sheet was studied firstly, and then the terahertz spectrum information of four cotton seeds in the effective frequency range was collected with the help of terahertz time-domain spectrometer. Furthermore, the characteristic information contained in the terahertz spectrum was qualitatively analyzed, and the terahertz absorption characteristics of four samples were preliminarily discussed. The results showed that the four cotton seeds showed different spectral response characteristics in terahertz band, especially in their absorption coefficient spectra. The three transgenic cotton varieties showed obvious absorption of terahertz at 1.21 ~ 1.23 and 1.41 THz. But the non-transgenic cotton seeds showed weak absorption or no absorption. These obvious differences suggest that the terahertz spectrum can be used as an effective tool to identify transgenic / non-transgenic cotton seeds, and this study can be used to further reveal the mechanism of terahertz spectrum detection. To develop the rapid detection method of transfer / non-GM substances to provide a reference.
【作者单位】：国家农业信息化工程技术研究中心;石河子大学机械电气工程学院;农业部农业信息技术重点实验室;
【基金】：国家自然科学基金项目(51405312)
【分类号】：S562

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