藏山羊FGF10基因克隆及其组织表达分析
发布时间：2018-11-14 21:24
【摘要】：为了探讨藏山羊成纤维细胞生长因子10(fibroblast growth factor 10,FGF10)基因分子结构特征,并阐明其在藏山羊不同器官组织中的表达情况,试验采用逆转录PCR(reverse transcription-PCR,RTPCR)克隆该基因,并采用实时荧光定量PCR(qPCR)技术检测FGF10基因在藏山羊各器官组织中的表达情况。结果表明:克隆获得藏山羊FGF10基因序列长度为1 252 bp,包括完整CDS区642 bp(Gen Bank登录号为KX530811),编码213个氨基酸,蛋白质分子质量为23.78 ku,属于疏水性不稳定蛋白,且主要分布于细胞外。与山羊FGF10基因CDS区比对结果显示,藏山羊FGF10基因序列存在一个核苷酸突变位点,并导致该位点缬氨酸(Val)突变为异亮氨酸(Ile)。组织表达结果显示,FGF10 mRNA主要在藏山羊肺脏、脾脏和背最长肌中高表达,而在心脏、肝脏和肾脏器官组织中表达水平较低。
[Abstract]:In order to investigate the molecular structure of the fibroblast growth factor 10 (fibroblast growth factor 10 FGF10 gene in Tibetan goats and to elucidate its expression in different organs of Tibetan goats, reverse transcription PCR (reverse transcription-PCR,RTPCR (RT PCR (reverse transcription-PCR,RTPCR) was used to clone the gene. The expression of FGF10 gene in organs and tissues of Tibetan goats was detected by real-time fluorescence quantitative PCR (qPCR). The results showed that the length of FGF10 gene of Tibetan goat was 1 252 bp, including the complete CDS region 642 bp (Gen Bank accession number was KX530811), encoding 213 amino acids, the molecular weight of protein was 23.78 ku, belongs to hydrophobic unstable protein. And mainly distributed outside cells. The comparison with the CDS region of goat FGF10 gene showed that there was a nucleotide mutation site in Tibetan goat FGF10 gene sequence, which led to the mutation of valine (Val) to isoleucine (Ile). The results of tissue expression showed that FGF10 mRNA was mainly expressed in lung, spleen and longissimus dorsi muscle of Tibetan goats, but low in heart, liver and kidney.
【作者单位】： 西南民族大学生命科学与技术学院;青藏高原动物遗传资源保护与利用四川省重点实验室;
【基金】：中央高校基本科研业务费专项资金项目(2016NY Z0045) 四川省“十三五”畜禽育种攻关项目(2016NZ0099-36) 四川省科技创新产业链示范工程重大项目(2014NZ0003)
【分类号】：S827
本文编号：2332369
[Abstract]:In order to investigate the molecular structure of the fibroblast growth factor 10 (fibroblast growth factor 10 FGF10 gene in Tibetan goats and to elucidate its expression in different organs of Tibetan goats, reverse transcription PCR (reverse transcription-PCR,RTPCR (RT PCR (reverse transcription-PCR,RTPCR) was used to clone the gene. The expression of FGF10 gene in organs and tissues of Tibetan goats was detected by real-time fluorescence quantitative PCR (qPCR). The results showed that the length of FGF10 gene of Tibetan goat was 1 252 bp, including the complete CDS region 642 bp (Gen Bank accession number was KX530811), encoding 213 amino acids, the molecular weight of protein was 23.78 ku, belongs to hydrophobic unstable protein. And mainly distributed outside cells. The comparison with the CDS region of goat FGF10 gene showed that there was a nucleotide mutation site in Tibetan goat FGF10 gene sequence, which led to the mutation of valine (Val) to isoleucine (Ile). The results of tissue expression showed that FGF10 mRNA was mainly expressed in lung, spleen and longissimus dorsi muscle of Tibetan goats, but low in heart, liver and kidney.
【作者单位】： 西南民族大学生命科学与技术学院;青藏高原动物遗传资源保护与利用四川省重点实验室;
【基金】：中央高校基本科研业务费专项资金项目(2016NY Z0045) 四川省“十三五”畜禽育种攻关项目(2016NZ0099-36) 四川省科技创新产业链示范工程重大项目(2014NZ0003)
【分类号】：S827
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，本文编号：2332369
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